Weisel Tamara, Baum Matthias, Eisenbrand Gerhard, Dietrich Helmut, Will Frank, Stockis Jean-Pierre, Kulling Sabine, Rüfer Corinna, Johannes Christian, Janzowski Christine
Department of Chemistry, Division of Food Chemistry and Environmental Toxicology, University of Kaiserslautern, Kaiserslautern, Germany.
Biotechnol J. 2006 Apr;1(4):388-97. doi: 10.1002/biot.200600004.
Oxidative cell damage is involved in the pathogenesis of atherosclerosis, cancer, diabetes and other diseases. Uptake of fruit juice with especially high content of antioxidant flavonoids/polyphenols, might reduce oxidative cell damage. Therefore, an intervention study was performed with a red mixed berry juice [trolox equivalent antioxidative capacity (TEAC): 19.1 mmol/L trolox] and a corresponding polyphenol-depleted juice (polyphenols largely removed, TEAC 2.4 mmol/L trolox), serving as control. After a 3-week run-in period, 18 male probands daily consumed 700 mL juice, and 9 consumed control juice, in a 4-week intervention, followed by a 3-week wash-out. Samples were collected weekly to analyze DNA damage (comet assay), lipid peroxidation (plasma malondialdehyde: HPLC/fluorescence; urinary isoprostanes: GC-MS), blood glutathione (photometrically), DNA-binding activity of nuclear factor-kappaB (ELISA) and plasma carotenoid/alpha-tocopherol levels (HPLC-DAD). During intervention with the fruit juice, a decrease of oxidative DNA damage (p<5x10(-4)) and an increase of reduced glutathione (p<5x10(-4)) and of glutathione status (p<0.05) were observed, which returned to the run-in levels in the subsequent wash-out phase. The other biomarkers were not significantly modulated by the juice supplement. Intervention with the control juice did not result in reduction of oxidative damage. In conclusion, the fruit juice clearly reduces oxidative cell damage in healthy probands.
氧化细胞损伤参与动脉粥样硬化、癌症、糖尿病及其他疾病的发病机制。摄入富含抗氧化黄酮类化合物/多酚的果汁可能会减少氧化细胞损伤。因此，开展了一项干预研究，使用一种红色混合浆果果汁[特洛克斯等效抗氧化能力（TEAC）：19.1 mmol/L特洛克斯]和一种相应的多酚耗尽果汁（多酚基本去除，TEAC 2.4 mmol/L特洛克斯）作为对照。在为期3周的导入期后，18名男性受试者在为期4周的干预中每天饮用700 mL果汁，9名受试者饮用对照果汁，随后是为期3周的洗脱期。每周采集样本以分析DNA损伤（彗星试验）、脂质过氧化（血浆丙二醛：HPLC/荧光法；尿异前列腺素：GC-MS）、血液谷胱甘肽（光度法）、核因子-κB的DNA结合活性（ELISA）以及血浆类胡萝卜素/α-生育酚水平（HPLC-DAD）。在饮用果汁干预期间，观察到氧化DNA损伤减少（p<5×10⁻⁴）、还原型谷胱甘肽增加（p<5×10⁻⁴）以及谷胱甘肽状态改善（p<0.05），在随后的洗脱期这些指标恢复到导入期水平。其他生物标志物未因补充果汁而受到显著调节。饮用对照果汁的干预未导致氧化损伤减少。总之，该果汁明显减少了健康受试者的氧化细胞损伤。
