Spormann Thomas M, Albert Franz W, Rath Thomas, Dietrich Helmut, Will Frank, Stockis Jean-Pierre, Eisenbrand Gerhard, Janzowski Christine
Department of Chemistry, Division of Food Chemistry and Toxicology, University of Kaiserslautern, Erwin-Schroedinger-Strasse 52, 67663 Kaiserslautern, Germany.
Cancer Epidemiol Biomarkers Prev. 2008 Dec;17(12):3372-80. doi: 10.1158/1055-9965.EPI-08-0364.
Hemodialysis patients face an elevated risk of cancer, arteriosclerosis, and other diseases, ascribed in part to increased oxidative stress. Red fruit juice with high anthocyanin/polyphenol content had been shown to reduce oxidative damage in healthy probands. To test its preventive potential in hemodialysis patients, 21 subjects in a pilot intervention study consumed 200 mL/day of red fruit juice (3-week run-in; 4-week juice uptake; 3-week wash-out). Weekly blood sampling was done to monitor DNA damage (comet assay +/- formamidopyrimidine-DNA glycosylase enzyme), glutathione, malondialdehyde, protein carbonyls, trolox equivalent antioxidant capacity, triglycerides, and DNA binding capacity of the transcription factor nuclear factor-kappaB. Results show a significant decrease of DNA oxidation damage (P < 0.0001), protein and lipid peroxidation (P < 0.0001 and P < 0.001, respectively), and nuclear factor-kappaB binding activity (P < 0.01), and an increase of glutathione level and status (both P < 0.0001) during juice uptake. We attribute this reduction in oxidative (cell) damage in hemodialysis patients to the especially high anthocyanin/polyphenol content of the juice. This provides promising perspectives into the prevention of chronic diseases such as cancer and cardiovascular disease in population subgroups exposed to enhanced oxidative stress like hemodialysis patients.
血液透析患者面临患癌症、动脉硬化及其他疾病的风险升高，部分原因是氧化应激增加。高花青素/多酚含量的红果汁已被证明可减少健康受试者的氧化损伤。为测试其对血液透析患者的预防潜力，一项初步干预研究中的21名受试者每天饮用200毫升红果汁（3周导入期；4周饮汁期；3周洗脱期）。每周采集血样以监测DNA损伤（彗星试验+/-甲酰胺嘧啶-DNA糖基化酶）、谷胱甘肽、丙二醛、蛋白质羰基、Trolox等效抗氧化能力、甘油三酯以及转录因子核因子-κB的DNA结合能力。结果显示，在饮汁期间，DNA氧化损伤（P < 0.0001）、蛋白质和脂质过氧化（分别为P < 0.0001和P < 0.001）以及核因子-κB结合活性（P < 0.01）显著降低，谷胱甘肽水平和状态升高（均为P < 0.0001）。我们将血液透析患者氧化（细胞）损伤的这种降低归因于果汁中特别高的花青素/多酚含量。这为预防诸如癌症和心血管疾病等慢性疾病提供了有前景的视角，这些慢性疾病在像血液透析患者这样暴露于增强氧化应激的人群亚组中存在。
