人气管黏膜中树突状细胞的密度与年龄相关且具有部位特异性。
Density of dendritic cells in the human tracheal mucosa is age dependent and site specific.
作者信息
Tschernig T, de Vries V C, Debertin A S, Braun A, Walles T, Traub F, Pabst R
机构信息
Functional and Applied Anatomy, Medical School of Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
出版信息
Thorax. 2006 Nov;61(11):986-91. doi: 10.1136/thx.2006.060335. Epub 2006 Aug 7.
BACKGROUND
The mucosal immune system undergoes extensive changes in early childhood in response to environmental stimuli. Dendritic cells (DC) play a major role in the development of the immune system. However, few data exist on the influence of continuous environmental stimulation on the distribution and phenotype of human airway DC.
METHODS
Human tissue samples are mostly paraffin embedded which limits the use of several antibodies, and respiratory tissue for cryopreservation is difficult to obtain. Human frozen post mortem tracheal tissue was therefore used for this study. Only samples with epithelial adherence to the basement membrane were included (n = 34). Immunohistochemical staining and sequential overlay immunofluorescence were performed with DC-SIGN and a panel of leucocyte markers co-expressed by DC.
RESULTS
DC detected in the human tracheal mucosa using DC-SIGN correlated with the expression of HLA-DR, co-stimulatory and adhesion molecules. Higher cell densities were found at the ventral tracheal site of patients older than 1 year than in infants in the first year of life.
CONCLUSION
The increasing population of mucosal DC with age could reflect immunological maturation.
背景
在儿童早期，黏膜免疫系统会因环境刺激而发生广泛变化。树突状细胞（DC）在免疫系统发育中起主要作用。然而，关于持续环境刺激对人气道DC分布和表型影响的数据很少。
方法
人体组织样本大多为石蜡包埋，这限制了几种抗体的使用，且难以获得用于冷冻保存的呼吸组织。因此，本研究使用了人类死后冷冻气管组织。仅纳入上皮附着于基底膜的样本（n = 34）。用DC-SIGN和DC共表达的一组白细胞标志物进行免疫组织化学染色和连续叠加免疫荧光。
结果
使用DC-SIGN在人气管黏膜中检测到的DC与HLA-DR、共刺激分子和黏附分子的表达相关。1岁以上患者气管腹侧部位的细胞密度高于1岁以内婴儿。
结论
黏膜DC数量随年龄增加可能反映免疫成熟。
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