Svenstrup Helle Friis, Jensen Jørgen Skov, Gevaert Kris, Birkelund Svend, Christiansen Gunna
Institute of Medical Microbiology and Immunology, The Bartholin Building, Aarhus University, DK-8000 Aarhus C, Denmark.
Clin Vaccine Immunol. 2006 Aug;13(8):913-22. doi: 10.1128/CVI.00048-06.
Mycoplasma genitalium causes nonchlamydial nongonococcal urethritis. M. genitalium was detected by PCR in 17 urethral swabs obtained from 99 men with and without urethritis (J. S. Jensen, R. Orsum, B. Dohn, S. Uldum, A. M. Worm, and K. Lind, Genitourin. Med. 69:265-269, 1993), and later, four M. genitalium strains were isolated (J. S. Jensen, H. T. Hansen, and K. Lind, J. Clin. Microbiol. 34:286-291, 1996). The objective of this study was to characterize immunogenic proteins of M. genitalium by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting by using a hyperimmune rabbit serum against M. genitalium G37, determine their identity by mass spectrometry, and develop an M. genitalium-specific enzyme-linked immunosorbent assay (ELISA) free from cross-reactivity with M. pneumoniae antibodies. Using recombinant fragments of the C-terminal part of MgPa (rMgPa), we developed a specific ELISA for detection of M. genitalium antibodies. This antigen did not bind M. pneumoniae antibodies. Using serum samples from the 99 men with and without urethritis, we found that 26 had immunoglobulin G (IgG) antibodies to M. genitalium. There was a strong statistically significant correlation between PCR and IgG antibodies to M. genitalium (odds ratio [OR], 5.9; 95% confidence interval [CI], 2.3 to 21.5; P = 0.002). Furthermore, men with recurrent urethritis were more likely to have antibodies to M. genitalium than were those without recurrent urethritis (OR, 4.0; 95% CI, 1.1 to 14.5; P = 0.0383) and they had significantly higher antibody titers. By use of the rMgPa ELISA, this study further substantiates the importance of M. genitalium as a cause of male urethritis.
生殖支原体可引起非衣原体性非淋菌性尿道炎。通过聚合酶链反应（PCR）在从99名患有或未患尿道炎的男性中获取的17份尿道拭子中检测到了生殖支原体（J.S.詹森、R.奥尔苏姆、B.多恩、S.乌尔杜姆、A.M.沃姆和K.林德，《泌尿生殖医学》69:265 - 269，1993年），随后，分离出了4株生殖支原体菌株（J.S.詹森、H.T.汉森和K.林德，《临床微生物学杂志》34:286 - 291，1996年）。本研究的目的是通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳和使用针对生殖支原体G37的超免疫兔血清进行免疫印迹来鉴定生殖支原体的免疫原性蛋白，通过质谱确定其身份，并开发一种与肺炎支原体抗体无交叉反应的生殖支原体特异性酶联免疫吸附测定（ELISA）。使用MgPa C末端部分的重组片段（rMgPa），我们开发了一种用于检测生殖支原体抗体的特异性ELISA。该抗原不结合肺炎支原体抗体。使用来自这99名患有或未患尿道炎男性的血清样本，我们发现26人有针对生殖支原体的免疫球蛋白G（IgG）抗体。PCR与生殖支原体IgG抗体之间存在高度统计学显著相关性（优势比[OR]，5.9；95%置信区间[CI]，2.3至21.5；P = 0.002）。此外，复发性尿道炎男性比无复发性尿道炎男性更有可能有针对生殖支原体的抗体（OR，4.0；95%CI，1.1至14.5；P = 0.0383），并且他们的抗体滴度显著更高。通过使用rMgPa ELISA，本研究进一步证实了生殖支原体作为男性尿道炎病因的重要性。
