利用 ESI-MS/CID 鉴定枯草芽孢杆菌中的丰原菌素同源物。
Identification of fengycin homologues from Bacillus subtilis with ESI-MS/CID.
机构信息
College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, 1 Weigang Nanjing 210095, PR China.
出版信息
J Microbiol Methods. 2009 Dec;79(3):272-8. doi: 10.1016/j.mimet.2009.09.013. Epub 2009 Sep 23.
Bacillus subtilis fmbJ (CGMCC no.0943) was used to produce lipopeptides antibiotics fengycin. The fengycin mixture was precipitated with 6N HCl and extracted by methanol. Electrospray ionization mass spectrometry/collision-induced dissociation (ESI-MS/CID) were used to analyze the fengycin mixture. In the ESI-MS/CID spectra of fengycin molecular ions, production ions (m/z 1080, m/z 966, m/z 1108 and m/z 994) were always detected. Therefore, these production ions (m/z 1080, m/z 966, m/z 1108 and m/z 994) could be used as fingerprints to quickly detect fengycin A and fengycin B. By the method of ESI-MS/CID, six homologues of fengycin A with C14 to 19 and four homologues of fengycin B with C14 to 17 were characterized. The method will be useful for rapidly determining whether a strain produces fengycin homologues.
枯草芽孢杆菌 fmbJ（CGMCC 编号 0943）被用于生产脂肽类抗生素丰原素。丰原素混合物用 6N HCl 沉淀并用甲醇提取。电喷雾电离质谱/碰撞诱导解离（ESI-MS/CID）被用于分析丰原素混合物。在丰原素分子离子的 ESI-MS/CID 谱中，总是检测到产物离子（m/z 1080、m/z 966、m/z 1108 和 m/z 994）。因此，这些产物离子（m/z 1080、m/z 966、m/z 1108 和 m/z 994）可以用作快速检测丰原素 A 和丰原素 B 的指纹。通过 ESI-MS/CID 方法，鉴定了六种丰原素 A 的同系物，它们的 C14 到 19，以及四种丰原素 B 的同系物，它们的 C14 到 17。该方法将有助于快速确定菌株是否产生丰原素同系物。
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