Aguiar Aderbal S, Tuon Talita, Soares Fernanda S, da Rocha Luís Gustavo C, Silveira Paulo César, Pinho Ricardo A
Laboratory of Exercise Physiology, PPGCS, Universidade do Extremo Sul Catarinense, Criciuma, SC 88806-000, Brazil.
Neurochem Res. 2008 May;33(5):729-36. doi: 10.1007/s11064-007-9485-8. Epub 2007 Oct 17.
The aim of this study was to analyze the effects of intense exercise on brain redox status, associated with antioxidant supplementation of N-acetylcysteine (NAC), deferoxamine (DFX) or a combination of both. Seventy-two C57BL-6 adult male mice were randomly assigned to 8 groups: control, NAC, DFX, NAC plus DFX, exercise, exercise with NAC, exercise with DFX, and exercise with NAC plus DFX. They were given antioxidant supplementation, exercise training on a treadmill for 12 weeks, and sacrificed 48 h after the last exercise session. Training significantly increased (P < 0.05) soleus citrate synthase (CS) activity when compared to control. Blood lactate levels classified the exercise as intense. Exercise significantly increased (P < 0.05) oxidation of biomolecules and superoxide dismutase activity in striatum and hippocampus. Training significantly increased (P < 0.05) catalase activity in striatum. NAC and DFX supplementation significantly protected (P < 0.05) against oxidative damage. These results indicate intense exercise as oxidant and NAC and DFX as antioxidant to the hippocampus and the striatum.
本研究旨在分析高强度运动对脑氧化还原状态的影响，以及与补充抗氧化剂N-乙酰半胱氨酸（NAC）、去铁胺（DFX）或两者联合使用的相关性。72只C57BL-6成年雄性小鼠被随机分为8组：对照组、NAC组、DFX组、NAC加DFX组、运动组、运动加NAC组、运动加DFX组、运动加NAC加DFX组。给予它们抗氧化剂补充，在跑步机上进行12周的运动训练，并在最后一次运动训练后48小时处死。与对照组相比，训练显著增加了（P<0.05）比目鱼肌柠檬酸合酶（CS）活性。血乳酸水平将该运动归类为高强度运动。运动显著增加了（P<0.05）纹状体和海马体中生物分子的氧化以及超氧化物歧化酶活性。训练显著增加了（P<0.05）纹状体中的过氧化氢酶活性。补充NAC和DFX显著保护（P<0.05）免受氧化损伤。这些结果表明高强度运动对海马体和纹状体具有氧化作用，而NAC和DFX具有抗氧化作用。
