基于绿色荧光蛋白（GFP）的显微镜技术中的纳米级分辨率。

Nanoscale resolution in GFP-based microscopy.

作者信息

Willig Katrin I, Kellner Robert R, Medda Rebecca, Hein Birka, Jakobs Stefan, Hell Stefan W

机构信息

Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.

出版信息

Nat Methods. 2006 Sep;3(9):721-3. doi: 10.1038/nmeth922.

DOI:10.1038/nmeth922

PMID:16896340

Abstract

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.

摘要

我们报告了使用受激辐射损耗（STED）显微镜对绿色荧光蛋白（GFP）标记样本实现亚衍射分辨率的情况。通过对GFP标记的病毒和哺乳动物细胞的内质网（ER）进行成像，证明了本研究中获得的约70纳米横向分辨率。我们的结果标志着具有基因编码标记的纳米级生物显微镜的问世。