Cases Nuria, Sureda Antoni, Maestre Isabel, Tauler Pedro, Aguiló Antoni, Córdova Alfredo, Roche Enrique, Tur Josep A, Pons Antoni
Departament de Biologia Fonamental i Ciències de la Salut, Edifici Guillem Colom, Universitat de les Illes Balears, Crta de Valldemossa, Palma de Mallorca, Balears, Spain.
Eur J Appl Physiol. 2006 Oct;98(3):263-9. doi: 10.1007/s00421-006-0273-y. Epub 2006 Aug 9.
The response of lymphocyte and plasma antioxidant defences to a prolonged exercise as a cycling stage in a professional race was analysed. Antioxidant enzyme activities and gene expression, carbonyl derivative and MDA levels were determined in lymphocytes. Plasma levels of vitamin E, carotenes, protein carbonyl derivatives and the test d-Roms were measured. Significant increases in plasmatic carbonyls and in the test d-Roms were observed after the cycling stage. No significant differences were found in the lymphocyte MDA and carbonyl derivative levels. A significant increase was found in plasma vitamin E concentration after the cycling stage; however, the lymphocyte vitamin E concentration did not change. Significant increases were observed in lymphocyte total superoxide dismutase (SOD) activity and in the levels of CuZn-SOD and Mn-SOD isoenzymes. The moderate levels of oxidative stress in the lymphocyte induced a cellular adaptation to exercise enough to counteract the negative effects of oxidative stress.
分析了淋巴细胞和血浆抗氧化防御系统对职业比赛中自行车赛段长时间运动的反应。测定了淋巴细胞中的抗氧化酶活性、基因表达、羰基衍生物和丙二醛水平。测量了血浆中维生素E、类胡萝卜素、蛋白质羰基衍生物和d-Roms检测指标的水平。自行车赛段后，血浆羰基和d-Roms检测指标显著增加。淋巴细胞丙二醛和羰基衍生物水平未发现显著差异。自行车赛段后血浆维生素E浓度显著增加；然而，淋巴细胞维生素E浓度没有变化。淋巴细胞总超氧化物歧化酶（SOD）活性以及铜锌超氧化物歧化酶和锰超氧化物歧化酶同工酶水平显著增加。淋巴细胞中适度的氧化应激水平诱导了细胞对运动的适应，足以抵消氧化应激的负面影响。
