细胞毒性T细胞识别疟原虫感染的肝细胞上环子孢子蛋白的一种肽段。
Cytotoxic T cells recognize a peptide from the circumsporozoite protein on malaria-infected hepatocytes.
作者信息
Weiss W R, Mellouk S, Houghten R A, Sedegah M, Kumar S, Good M F, Berzofsky J A, Miller L H, Hoffman S L
机构信息
Infectious Disease Department, Naval Medical Research Institute, Bethesda, Maryland 20814.
出版信息
J Exp Med. 1990 Mar 1;171(3):763-73. doi: 10.1084/jem.171.3.763.
Abstract
Irradiated malaria sporozoites can induce CD8+ T cells that are required for protection against infection. However, the parasite antigens targeted by this immune response are unknown. We have discovered a 16-amino acid epitope from the Plasmodium yoelii circumsporozoite (CS) protein that is recognized by cytotoxic T cells from immune mice. Lymphocytes stimulated with this peptide can kill P. yoelii liver stage parasites in vitro in an MHC-restricted, antigen-specific manner. Thus, epitopes from the CS protein are presented on the surface of infected hepatocytes and can be targets for T cells, even though intact CS protein has not been detected on the surface of the infected hepatocyte. A vaccine that induced CTL to parasite antigens might protect humans against malaria by eliminating liver stage parasites.
摘要
经辐照的疟原虫子孢子可诱导产生抵御感染所需的CD8+ T细胞。然而，这种免疫反应所靶向的寄生虫抗原尚不清楚。我们从约氏疟原虫环子孢子（CS）蛋白中发现了一个16个氨基酸的表位，该表位可被免疫小鼠的细胞毒性T细胞识别。用此肽刺激的淋巴细胞能够以MHC限制的、抗原特异性的方式在体外杀死约氏疟原虫肝期寄生虫。因此，尽管在受感染肝细胞表面未检测到完整的CS蛋白，但CS蛋白的表位会呈现在受感染肝细胞表面，并且可成为T细胞的靶标。一种能诱导CTL针对寄生虫抗原的疫苗或许可通过清除肝期寄生虫来保护人类免受疟疾侵害。
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本文引用的文献
1
[In vitro infestation of adult thamnomys hepatocytes with Plasmodium yoelii sporozoites; schizogony and release of infecting merozoites].[约氏疟原虫子孢子对成年非洲攀鼠肝细胞的体外感染；裂体生殖及感染性裂殖子的释放]
C R Seances Acad Sci III. 1982 May 24;294(19):963-5.
2
Serological reactivity of in vitro cultured exoerythrocytic stages of Plasmodium berghei in indirect immunofluorescent or immunoperoxidase antibody tests.
Am J Trop Med Hyg. 1983 Jan;32(1):24-30. doi: 10.4269/ajtmh.1983.32.24.
3
4
Protective immunity produced by the injection of x-irradiated sporozoites of plasmodium berghei.注射经X射线照射的伯氏疟原虫子孢子产生的保护性免疫。
Nature. 1967 Oct 14;216(5111):160-2. doi: 10.1038/216160a0.
5
Immunization of man against sporozite-induced falciparum malaria.人类针对子孢子诱导的恶性疟原虫疟疾的免疫接种。
Am J Med Sci. 1973 Sep;266(3):169-77. doi: 10.1097/00000441-197309000-00002.
6
Hydrophobicity scales and computational techniques for detecting amphipathic structures in proteins.用于检测蛋白质中两亲性结构的疏水性标度和计算技术。
J Mol Biol. 1987 Jun 5;195(3):659-85. doi: 10.1016/0022-2836(87)90189-6.
7
Plasmodium berghei: cloning of the circumsporozoite protein gene.伯氏疟原虫：环子孢子蛋白基因的克隆
Exp Parasitol. 1987 Jun;63(3):295-300. doi: 10.1016/0014-4894(87)90176-7.
8
Effect of antibodies to recombinant and synthetic peptides on P. falciparum sporozoites in vitro.抗重组和合成肽抗体对恶性疟原虫子孢子的体外作用。
Science. 1986 Jan 10;231(4734):156-9. doi: 10.1126/science.3510455.
9
A liver-stage-specific antigen of Plasmodium falciparum characterized by gene cloning.一种通过基因克隆鉴定的恶性疟原虫肝期特异性抗原。
Nature. 1987;329(6135):164-7. doi: 10.1038/329164a0.
10
The fate of the circumsporozoite antigens during the exoerythrocytic stage of Plasmodium berghei.
Eur J Cell Biol. 1988 Apr;46(1):25-30.
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