Guerin-Marchand C, Druilhe P, Galey B, Londono A, Patarapotikul J, Beaudoin R L, Dubeaux C, Tartar A, Mercereau-Puijalon O, Langsley G
Nature. 1987;329(6135):164-7. doi: 10.1038/329164a0.
The liver phase of development of malaria parasites has been studied only recently and remains poorly understood compared to the other stages such as sporozoïtes, merozoïtes and gametes. Access to liver forms of Plasmodium falciparum has been improved by the development of in vivo and in vitro propagation methods, but the yield of mature schizonts remains limited and does not allow a detailed antigenic analysis. To date, only immunofluorescence assays (IFA) have permitted a description of a species and liver-stage-specific antigen(s) (LSA; ref. 3). Monospecific antibodies to these antigens have not been obtained due either to difficulty in immunizing mice (against LSA), or to poor stability of human monoclonal antibodies. Therefore, as a means of characterizing the LSA, we used an alternative immunological approach to identify clones of the corresponding LSA genes. We describe here the isolation of a DNA sequence coding for a P. falciparum liver-stage-specific antigen composed of repeats of 17 amino-acids, which is immunogenic in man.
疟原虫发育的肝脏阶段直到最近才被研究，与其他阶段如子孢子、裂殖子和配子相比，人们对其了解仍然很少。通过体内和体外繁殖方法的发展，获取恶性疟原虫肝脏形式的途径得到了改善，但成熟裂殖体的产量仍然有限，无法进行详细的抗原分析。迄今为止，只有免疫荧光测定法（IFA）能够描述一种物种和肝脏阶段特异性抗原（LSA；参考文献3）。由于免疫小鼠存在困难（针对LSA）或人单克隆抗体稳定性差，尚未获得针对这些抗原的单特异性抗体。因此，作为表征LSA的一种方法，我们使用了一种替代免疫方法来鉴定相应LSA基因的克隆。我们在此描述了一个编码由17个氨基酸重复组成的恶性疟原虫肝脏阶段特异性抗原的DNA序列的分离，该抗原在人体内具有免疫原性。
