Bibikova Marina, Chudin Eugene, Wu Bonnie, Zhou Lixin, Garcia Eliza Wickham, Liu Ying, Shin Soojung, Plaia Todd W, Auerbach Jonathan M, Arking Dan E, Gonzalez Rodolfo, Crook Jeremy, Davidson Bruce, Schulz Thomas C, Robins Allan, Khanna Aparna, Sartipy Peter, Hyllner Johan, Vanguri Padmavathy, Savant-Bhonsale Smita, Smith Alan K, Chakravarti Aravinda, Maitra Anirban, Rao Mahendra, Barker David L, Loring Jeanne F, Fan Jian-Bing
Illumina, Inc., San Diego, California 92121, USA.
Genome Res. 2006 Sep;16(9):1075-83. doi: 10.1101/gr.5319906. Epub 2006 Aug 9.
Human embryonic stem (hES) cells originate during an embryonic period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self-renewal and pluripotence. We compared the DNA methylation status of 1536 CpG sites (from 371 genes) in 14 independently isolated hES cell lines with five other cell types: 24 cancer cell lines, four adult stem cell populations, four lymphoblastoid cell lines, five normal human tissues, and an embryonal carcinoma cell line. We found that the DNA methylation profile clearly distinguished the hES cells from all of the other cell types. A subset of 49 CpG sites from 40 genes contributed most to the differences among cell types. Another set of 25 sites from 23 genes distinguished hES cells from normal differentiated cells and can be used as biomarkers to monitor differentiation. Our results indicate that hES cells have a unique epigenetic signature that may contribute to their developmental potential.
人类胚胎干细胞（hES）起源于胚胎时期活跃的表观遗传重塑阶段。DNA甲基化模式可能对其自我更新和多能性至关重要。我们比较了14个独立分离的hES细胞系中1536个CpG位点（来自371个基因）的DNA甲基化状态，以及其他五种细胞类型：24个癌细胞系、四个成体干细胞群体、四个淋巴母细胞系、五种正常人体组织和一个胚胎癌细胞系。我们发现，DNA甲基化谱能够清晰地将hES细胞与所有其他细胞类型区分开来。来自40个基因的49个CpG位点的一个子集对细胞类型之间的差异贡献最大。来自23个基因的另一组25个位点将hES细胞与正常分化细胞区分开来，可作为监测分化的生物标志物。我们的结果表明，hES细胞具有独特的表观遗传特征，这可能有助于其发育潜能。
