Das Kusal K, Gupta Amrita Das, Dhundasi Salim A, Patil Ashok M, Das Swastika N, Ambekar Jeevan G
Department of Physiology, Al-Ameen Medical College, Bijapur, 586108, Karnataka, India.
Biometals. 2007 Apr;20(2):177-84. doi: 10.1007/s10534-006-9025-z. Epub 2006 Aug 10.
In this experimental study, we investigated whether L-ascorbic acid has any influence on the blood antioxidant defense system, lipid peroxidation and hematological parameters of the albino rats exposed to nickel sulfate(NiSO4). Twenty four adult rats were divided into four groups of six animals in each group. The control rats were untreated and comprised Group I. Group II rats were administered nickel sulfate (2.0 mg/100 g b.wt.; intraperitonially, i.p.). Group II rats were treated orally L-ascorbic acid (50 mg/100 g b.wt.) and Group IV rats were given both nickel sulfate and L-ascorbic acid simultaneously on alternate days until the tenth dose. The hematological parameters were assessed: red blood corpuscle counts, packed cell volume %, hemoglobin concentration, white blood corpuscle counts and platelets count decreased significantly and clotting time increased significantly in nickel treated rats. We also observed increase malondialdehyde (MDA) and decrease glutathione level (GSH) in erythrocytes of nickel treated rats. The activities of erythrocyte antioxidant enzymes like superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were significantly increased in rats treated with nickel sulfate. Simultaneously treatment of L-ascorbic acid exhibited a possible protective role on the toxic effect of nickel sulfate on the hematological values, erythrocyte MDA and GSH concentrations as well as antioxidant enzymatic defense system.
在本实验研究中，我们调查了L-抗坏血酸对暴露于硫酸镍（NiSO4）的白化大鼠的血液抗氧化防御系统、脂质过氧化和血液学参数是否有任何影响。24只成年大鼠被分为四组，每组6只动物。对照组大鼠未接受处理，为第一组。第二组大鼠腹腔注射硫酸镍（2.0 mg/100 g体重）。第三组大鼠口服L-抗坏血酸（50 mg/100 g体重），第四组大鼠每隔一天同时给予硫酸镍和L-抗坏血酸，直至第十剂。评估血液学参数：经镍处理的大鼠红细胞计数、血细胞压积百分比、血红蛋白浓度、白细胞计数和血小板计数显著降低，凝血时间显著增加。我们还观察到经镍处理的大鼠红细胞中丙二醛（MDA）增加，谷胱甘肽水平（GSH）降低。经硫酸镍处理的大鼠红细胞抗氧化酶如超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）的活性显著增加。同时给予L-抗坏血酸对硫酸镍对血液学值、红细胞MDA和GSH浓度以及抗氧化酶防御系统的毒性作用表现出可能的保护作用。
