在现场条件下评估巴氏染色法用于研究颊细胞中的微核。
Evaluation of Papanicolaou stain for studying micronuclei in buccal cells under field conditions.
作者信息
Ayyad Sohair B A, Israel Ebenezer, El-Setouhy Maged, Nasr Ghada Radwan, Mohamed Mostafa K, Loffredo Christopher A
机构信息
Cytopathology and Early Cancer Detection Unit, Ain Shams University Hospitals, Egypt.
出版信息
Acta Cytol. 2006 Jul-Aug;50(4):398-402. doi: 10.1159/000325980.
OBJECTIVE
To compare Papanicolaou (Pap) and May-Grünwald Giemsa (MGG) stain as 2 techniques for staining for buccal mucosal cells to detect micronuclei (MN) infield studies.
STUDY DESIGN
Eighty cytologic smears (2 per individual) were taken from the buccal mucosa of 40 cigarette smokers recruited at a rural village in Egypt. Forty smears were stained with Pap stain and 40 with MGG stain. All were assessed for cellularity and scored for MN.
RESULTS
Pap stain was faster and easier to process and transport in the field study than was MGG stain. Regarding MGG smears, bacteria and cell debris masked the MN as compared to Pap smears, in which the fixative destroyed the bacteria and made the cell boundaries clearly demarcated. Using Pap stain, MN were seen easily in transparent cytoplasm.
CONCLUSION
Pap stain is the preferred method infield studies for scoring and detecting MN in cells of buccal mucosa.
目的
比较巴氏染色（Pap）和迈-格鲁恩瓦尔德吉姆萨染色（MGG）作为两种用于颊黏膜细胞染色以在现场研究中检测微核（MN）的技术。
研究设计
从埃及一个乡村招募的40名吸烟者的颊黏膜采集了80份细胞学涂片（每人2份）。40份涂片用巴氏染色，40份用MGG染色。所有涂片均评估细胞数量并对微核进行评分。
结果
在现场研究中，巴氏染色比MGG染色处理和运输更快、更容易。对于MGG涂片，与巴氏涂片相比，细菌和细胞碎片掩盖了微核，在巴氏涂片中，固定剂破坏了细菌并使细胞边界清晰可辨。使用巴氏染色，微核很容易在透明的细胞质中看到。
结论
巴氏染色是现场研究中对颊黏膜细胞微核进行评分和检测的首选方法。
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