Montgelard C, Benyamin Y, Roustan C
Laboratoire de Paléontologie des Vertébrés EPHE, Institut des Sciences de l'Evolution CNRS (UA 327), USTL, Montpellier, France.
Experientia. 1990 Mar 15;46(3):303-7. doi: 10.1007/BF01951773.
Three closely related species of short-tailed mice (Mus musculus musculus, M. spretoides and M. spicilegus) were tentatively discriminated using immunological techniques based on albumin cross-reactivity. Different fractionations of crude albumin antisera allowed the recovery of antibody populations specific to the M. m. musculus albumin, whereas antibody population differences do not seem to exist between M. spicilegus and M. spretoides. Moreover, immunoreactivities tested with native and S-carboxymethylated albumins revealed that species-specific antibodies correspond to antigenic determinants depending on the amino acid sequence (sequential determinants). The observed immunological differences are related to species divergence and albumin sequences.
基于白蛋白交叉反应性，运用免疫技术初步区分了三种亲缘关系密切的短尾小鼠（小家鼠指名亚种、斯氏小家鼠和罗马尼亚小家鼠）。粗制白蛋白抗血清的不同分级分离能够分离出针对小家鼠指名亚种白蛋白的特异性抗体群体，而罗马尼亚小家鼠和斯氏小家鼠之间似乎不存在抗体群体差异。此外，用天然白蛋白和S-羧甲基化白蛋白进行的免疫反应性测试表明，物种特异性抗体对应于取决于氨基酸序列的抗原决定簇（顺序决定簇）。观察到的免疫差异与物种分化和白蛋白序列有关。
