Ang Eugenius S B C, Gluncic Vicko, Duque Alvaro, Schafer Mark E, Rakic Pasko
Department of Neurobiology and Kavli Institute for Neuroscience, Yale Medical School, Sterling Hall of Medicine, Room C-318, 333 Cedar Street, New Haven, CT 06510, USA.
Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12903-10. doi: 10.1073/pnas.0605294103. Epub 2006 Aug 10.
Neurons of the cerebral neocortex in mammals, including humans, are generated during fetal life in the proliferative zones and then migrate to their final destinations by following an inside-to-outside sequence. The present study examined the effect of ultrasound waves (USW) on neuronal position within the embryonic cerebral cortex in mice. We used a single BrdU injection to label neurons generated at embryonic day 16 and destined for the superficial cortical layers. Our analysis of over 335 animals reveals that, when exposed to USW for a total of 30 min or longer during the period of their migration, a small but statistically significant number of neurons fail to acquire their proper position and remain scattered within inappropriate cortical layers and/or in the subjacent white matter. The magnitude of dispersion of labeled neurons was variable but systematically increased with duration of exposure to USW. These results call for a further investigation in larger and slower-developing brains of non-human primates and continued scrutiny of unnecessarily long prenatal ultrasound exposure.
包括人类在内的哺乳动物大脑新皮质中的神经元，在胎儿期于增殖区产生，然后按照由内向外的顺序迁移至其最终位置。本研究检测了超声波（USW）对小鼠胚胎大脑皮质内神经元位置的影响。我们通过单次注射BrdU来标记在胚胎第16天产生并注定迁移至皮质浅层的神经元。我们对335只以上动物的分析表明，在神经元迁移期间，当总共暴露于超声波30分钟或更长时间时，一小部分但具有统计学意义的神经元未能获得其正确位置，而是散落在不适当的皮质层和/或相邻白质中。标记神经元的分散程度各不相同，但随着暴露于超声波时间的延长而系统性增加。这些结果需要在更大且发育较慢的非人类灵长类动物大脑中进行进一步研究，并持续审查不必要的长时间产前超声暴露情况。
