Biomaterials and Biocatalysts, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604.
Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641 003, Tamilnadu, India.
Int J Syst Evol Microbiol. 2014 Jun;64(Pt 6):2023-2028. doi: 10.1099/ijs.0.055525-0. Epub 2014 Mar 20.
A novel, yellow-pigmented bacterium, designated strain MO64(T), was isolated from the rhizoplane of field-grown soybean, collected from an experimental plot at Coimbatore, India. Cells were Gram-reaction-negative, motile, non-spore-forming rods that produced yellow-pigmented colonies on R2A agar. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain MO64(T) belonged to the genus Rhodanobacter. Strain MO64(T) was related most closely to Rhodanobacter ginsengisoli GR17-7(T) (98.0% 16S rRNA gene sequence similarity), Rhodanobacter spathiphylli B39(T) (97.9%), Rhodanobacter panaciterrae LnR5-47(T) (97.7%), Rhodanobacter terrae GP18-1(T) (97.6%), Rhodanobacter soli DCY45(T) (97.3%) and Rhodanobacter caeni MJ01(T) (97.2%); levels of similarity to the type strains of all other recognized species of the genus Rhodanobacter were less than 97.0%. Chemotaxonomic data (Q-8 as the predominant ubiquinone, and iso-C(16 : 0), iso-C(15 : 0), C(17 : 0) cyclo, iso-C(17 : 1)ω9c, iso-C(17 : 0) and iso-C(11 : 0) as the major fatty acids) also supported the affiliation of strain MO64(T) with the genus Rhodanobacter. The G+C content of the genomic DNA was 64 mol%. The results of DNA-DNA hybridization and phenotypic analysis showed that strain MO64(T) can be distinguished from all known species of the genus Rhodanobacter and therefore represents a novel species of the genus, for which the name Rhodanobacter glycinis sp. nov. is proposed. The type strain is MO64(T) ( = ICMP 17626(T) = NBRC 105007(T)).
一株新的黄色着色细菌，被命名为 MO64(T) 菌株，从印度哥印拜陀试验区田间生长的大豆根际中分离得到。细胞革兰氏反应阴性，可运动，不形成孢子的杆状，在 R2A 琼脂上产生黄色着色的菌落。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MO64(T) 属于 Rhodanobacter 属。菌株 MO64(T) 与 Rhodanobacter ginsengisoli GR17-7(T)（98.0% 16S rRNA 基因序列相似性）、Rhodanobacter spathiphylli B39(T)（97.9%）、Rhodanobacter panaciterrae LnR5-47(T)（97.7%）、Rhodanobacter terrae GP18-1(T)（97.6%）、Rhodanobacter soli DCY45(T)（97.3%）和 Rhodanobacter caeni MJ01(T)（97.2%）最为密切相关；与属内所有其他公认的种的模式菌株的相似性均低于 97.0%。化学分类数据（Q-8 为主要的泛醌，以及 iso-C(16:0)、iso-C(15:0)、C(17:0)cyclo、iso-C(17:1)ω9c、iso-C(17:0)和 iso-C(11:0)为主要脂肪酸）也支持菌株 MO64(T) 与 Rhodanobacter 属的归属。基因组 DNA 的 G+C 含量为 64 mol%。DNA-DNA 杂交和表型分析的结果表明，菌株 MO64(T) 可与 Rhodanobacter 属的所有已知种区分开来，因此代表了该属的一个新种，建议将其命名为 Rhodanobacter glycinis sp. nov.。模式菌株为 MO64(T)（=ICMP 17626(T)=NBRC 105007(T)）。
