根际黄杆菌属，新属，新种，一种从大麦（Hordeum vulgare L.）根际分离出的产黄色色素的γ-变形菌。
Luteibacter rhizovicinus gen. nov., sp. nov., a yellow-pigmented gammaproteobacterium isolated from the rhizosphere of barley (Hordeum vulgare L.).
作者信息
Johansen Jens E, Binnerup Svend J, Kroer Niels, Mølbak Lars
机构信息
Department of Environmental Chemistry and Microbiology, National Environmental Research Institute, Frederiksborgvej 399, DK-4000 Roskilde, Denmark.
Department of Bacteriology, Danish Veterinary Institute, Bülowsvej 27, DK-1790 København V, Denmark.
出版信息
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2285-2291. doi: 10.1099/ijs.0.63497-0.
Three strains of Gram-negative, aerobic, yellow-pigmented, chemo-organotrophic bacteria, motile by a polar flagellum, were isolated from the rhizosphere of spring barley (Hordeum vulgare L.) at a research field near Copenhagen, Denmark. The three strains, LJ79, LJ96T and LJ99, formed visible colonies on one-tenth-strength tryptic soy broth supplemented with agar (1/10 TSBA) after incubation for 6 days at 15 degrees C. The strains hydrolysed starch, casein (skimmed milk), gelatin and various pentoses and hexoses and grew on MacConkey agar and full-strength TSBA. Growth on 1/10 TSBA occurred at 4-30 degrees C, pH 6-9 and 0-3 % (w/v) NaCl. The strains had identical 16S rRNA gene sequences and ERIC (enterobacterial repetitive intergenic consensus sequence) fingerprint profiles, but could be differentiated by their RAPD (random amplified polymorphic DNA) fingerprint patterns. Strain LJ96(T) had a DNA G+C content of 64.3 mol% and the major fatty acids were 15 : 0 iso (23.4 %), 17 : 1 iso omega9c (25.5 %) and 17 : 0 iso (18.1 %). Phylogenetic analysis of the 16S rRNA gene sequences of the three strains showed 96 % sequence similarity to Rhodanobacter lindaniclasticus LMG 18385T, 95 % to Frateuria aurantia DSM 6220T and 96 % to Fulvimonas soli LMG 19981T. Using LJ96T DNA as probe, DNA-DNA hybridizations documented the relationship of the three strains to a single species (87.4-98.7 % relatedness) and showed less than 30 % relatedness to Frateuria aurantia DSM 6220T and Fulvimonas soli DSM 14263T. Rhodanobacter lindaniclasticus LMG 18385T is not extant and the strain not available from any public strain collections, thus DNA-DNA hybridization could not include this strain. On the basis of genotypic and phenotypic characteristics, the three yellow-pigmented strains could also be differentiated from Frateuria aurantia, Fulvimonas soli and Rhodanobacter lindaniclasticus. The name Luteibacter rhizovicinus gen. nov., sp. nov. is proposed, with the type strain LJ96T (=DSM 16549T=ATCC BAA-1015T).
从丹麦哥本哈根附近一个研究田的春大麦（Hordeum vulgare L.）根际分离出三株革兰氏阴性、需氧、产黄色色素、化能有机营养型细菌，它们通过极生鞭毛运动。这三株菌，LJ79、LJ96T和LJ99，在添加琼脂的十分之一强度胰蛋白胨大豆肉汤（1/10 TSBA）上于15℃培养6天后形成可见菌落。这些菌株能水解淀粉、酪蛋白（脱脂牛奶）、明胶以及各种戊糖和己糖，并且能在麦康凯琼脂和全强度TSBA上生长。在1/10 TSBA上生长的温度范围是4 - 30℃，pH值为6 - 9，NaCl浓度为0 - 3%（w/v）。这三株菌具有相同的16S rRNA基因序列和ERIC（肠杆菌重复基因间共有序列）指纹图谱，但可通过它们的RAPD（随机扩增多态性DNA）指纹图谱加以区分。菌株LJ96(T)的DNA G + C含量为64.3 mol%，主要脂肪酸为15 : 0异（23.4%）、17 : 1异ω9c（25.5%）和17 : 0异（18.1%）。对这三株菌的16S rRNA基因序列进行系统发育分析表明，它们与林丹降解红杆菌Rhodanobacter lindaniclasticus LMG 18385T的序列相似性为96%，与橙色发酵单胞菌Frateuria aurantia DSM 6220T的相似性为95%，与土壤富黄单胞菌Fulvimonas soli LMG 19981T的相似性为96%。以LJ96T DNA为探针进行的DNA - DNA杂交证明这三株菌属于同一个物种（相关性为87.4 - 98.7%），并且与橙色发酵单胞菌Frateuria aurantia DSM 6220T和土壤富黄单胞菌Fulvimonas soli DSM 14263T的相关性小于30%。林丹降解红杆菌Rhodanobacter lindaniclasticus LMG 18385T已不存在，且无法从任何公共菌株保藏中心获得该菌株，因此DNA - DNA杂交未包括此菌株。基于基因型和表型特征，这三株产黄色色素菌株也可与橙色发酵单胞菌、土壤富黄单胞菌和林丹降解红杆菌区分开来。提议将其命名为根际黄杆菌Luteibacter rhizovicinus gen. nov., sp. nov.，模式菌株为LJ96T（=DSM 16549T =ATCC BAA - 1015T）。
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