Weon Hang-Yeon, Kim Byung-Yong, Yoo Seung-Hee, Lee Seon-Young, Kwon Soon-Wo, Go Seung-Joo, Stackebrandt Erko
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea.
Int J Syst Evol Microbiol. 2006 Aug;56(Pt 8):1777-1782. doi: 10.1099/ijs.0.64242-0.
Two novel strains, GR20-10(T) and GR20-13(T), were isolated from soil using R2A medium. The soil sample was collected from a field in the Yeongju region of Korea that was cultivated with Korean ginseng. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains formed a cluster with several uncultured bacterial clones and with Flexibacter filiformis, Flexibacter sancti, Flexibacter japonensis, Cytophaga arvensicola and Flavobacterium ferrugineum (recently reclassified as Terrimonas ferruginea) in the phylum Bacteroidetes. The level of 16S rRNA gene sequence similarity between the two novel strains was 98.9 %. Isolates GR20-10(T) and GR20-13(T) showed the highest sequence similarities to Flexibacter japonensis IFO 16041(T) (91.8 and 91.9 %, respectively) and T. ferruginea ATCC 13524(T) (90.4 and 90.6 %, respectively). The whole-cell fatty acid profiles of the two isolates were similar and their major fatty acids were 15 : 0 iso, 17 : 0 iso 3-OH and 15 : 1 iso G. The major isoprenoid quinone of both strains was MK-7. The G+C contents of GR20-10(T) and GR20-13(T) were 45.8 and 44.3 mol%, respectively. DNA-DNA hybridization (57 % DNA-DNA hybridization value) and phenotypic data indicated that strains GR20-10(T) and GR20-13(T) each belong to a separate species. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains GR20-10(T) and GR20-13(T) represent two novel species in a novel genus in the phylum Bacteroidetes; the names Niastella koreensis gen. nov., sp. nov. (the type species; type strain GR20-10(T)=KACC 11465(T)=DSM 17620(T)) and Niastella yeongjuensis sp. nov. (type strain GR20-13(T)=KACC 11466(T)=DSM 17621(T)) are proposed.
使用R2A培养基从土壤中分离出两株新菌株GR20 - 10(T)和GR20 - 13(T)。土壤样本采自韩国永川地区种植高丽参的一块田地。基于16S rRNA基因序列的系统发育分析表明，这些菌株与拟杆菌门中的几个未培养细菌克隆以及丝状屈挠杆菌、圣洁屈挠杆菌、日本屈挠杆菌、田野生胞菌和铁锈黄杆菌（最近重新分类为铁锈地单胞菌）形成一个簇。两株新菌株之间的16S rRNA基因序列相似性水平为98.9%。分离株GR20 - 10(T)和GR20 - 13(T)与日本屈挠杆菌IFO 16041(T)（分别为91.8%和91.9%）以及铁锈地单胞菌ATCC 13524(T)（分别为90.4%和90.6%）显示出最高的序列相似性。这两株分离株的全细胞脂肪酸谱相似，其主要脂肪酸为15 : 0异戊酸、17 : 0异戊酸3 - OH和15 : 1异戊酸G。两株菌株的主要类异戊二烯醌均为MK - 7。GR20 - 10(T)和GR20 - 13(T)的G + C含量分别为45.8和44.3 mol%。DNA - DNA杂交（DNA - DNA杂交值为57%）和表型数据表明，菌株GR20 - 10(T)和GR20 - 13(T)各自属于一个独立的物种。基于表型和系统发育数据以及基因组独特性，菌株GR20 - 10(T)和GR20 - 13(T)代表拟杆菌门中一个新属的两个新物种；提出新属名韩国尼亚斯氏菌属（Niastella koreensis），新种名韩国尼亚斯氏菌（Niastella koreensis gen. nov., sp. nov.）（模式种；模式菌株GR20 - 10(T)=KACC 11465(T)=DSM 17620(T)）和新种名永川尼亚斯氏菌（Niastella yeongjuensis sp. nov.）（模式菌株GR20 - 13(T)=KACC 11466(T)=DSM 17621(T)）。
