Siddiqi Muhammad Zubair, Huq Md Amdadul, Im Wan-Taek
Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 17579, Republic of Korea.
AceEMzyme Co., Ltd, Academic Industry Cooperation, 327 Chungang-no, Anseong-si, Kyonggi-do, 17579, Republic of Korea.
Antonie Van Leeuwenhoek. 2021 Jan;114(1):11-22. doi: 10.1007/s10482-020-01485-4. Epub 2020 Nov 23.
A novel yellow-pigmented bacterial strain (designated BR5-29), was isolated and its taxonomy was studied. Phylogenetic study based on the 16S rRNA and draft genome sequence placed the strain BR5-29 in a distinct lineage within the family Chitinophagaceae, sharing ≤ 93.4% sequence similarity with members of the closely related genera Ferruginibacter, Flavisolibacter, Flavitalea and Niastella. The novel isolate showed the highest sequence similarity to the genus Ferruginibacter. The draft genome of strain BR5-29 had a total length of 5,505,520 base pairs. A total of 4585 genes were identified, in which 4537 were CDS and 48 RNA genes were assigned a putative function. The genome annotation of BR5-29 showed 225 carbohydrate genes which may be responsible for the conversion of major ginsenosides to minor ginsenosides. Strain BR5-29 contained MK-7 as a predominant quinone, and iso-C, iso-C G, iso-C 3-OH, and C ω7c and/or C ω6c (summed feature 3) as major fatty acids. The polar lipids found in the strain BR5-29 were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), five unidentified polar lipids (L1-L5), two unidentified aminolipid and one unidentified aminophospholipid. Our pilot data demonstrate that the novel isolate shares the similar major polar lipid PE, major quinone MK-7 and major fatty acids with the described members of the family Chitinophagaceae. However, the low 16S rRNA gene sequence (< 93.4%), the little high amount of C, iso-C 2-OH and iso-C 2-OH fatty acids, low DNA G + C content, and the presence of DPG, PG and two unidentified polar lipids (L1 and L3 differentiate the BR5-29 from its closest phylogenetic neighbors. Thus, the isolate represents a novel genus and species in the family Chitinophagaceae for which the name Ginsengibacter hankyongi gen. nov., sp. nov. is proposed. The type strain is BR5-29 (= KACC 19446 = LMG 30462). Thus, we predict that this novel strain may prove useful for the future research analysis (target gene cloning) and mass production of Rg3.
分离出一株新型黄色色素细菌菌株（命名为BR5-29），并对其进行了分类学研究。基于16S rRNA和草图基因组序列的系统发育研究将菌株BR5-29置于噬几丁质菌科内一个独特的谱系中，与密切相关的铁锈杆菌属、黄杆菌属、黄单胞菌属和泥生杆菌属成员的序列相似性≤93.4%。该新型分离株与铁锈杆菌属显示出最高的序列相似性。菌株BR5-29的草图基因组全长为5,505,520个碱基对。共鉴定出4585个基因，其中4537个为编码序列（CDS），48个RNA基因被赋予了推定功能。BR5-29的基因组注释显示有225个碳水化合物基因，可能负责将主要人参皂苷转化为次要人参皂苷。菌株BR5-29含有MK-7作为主要醌类，异C、异C G、异C 3-OH以及C ω7c和/或C ω6c（总和特征3）作为主要脂肪酸。在菌株BR5-29中发现的极性脂质有磷脂酰甘油（PG）、二磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）、五种未鉴定的极性脂质（L1-L5）、两种未鉴定的氨基脂质和一种未鉴定的氨基磷脂。我们的初步数据表明，该新型分离株与已描述的噬几丁质菌科成员具有相似的主要极性脂质PE、主要醌类MK-7和主要脂肪酸。然而，低16S rRNA基因序列（<93.4%）、少量高含量的C、异C 2-OH和异C 2-OH脂肪酸、低DNA G+C含量以及DPG、PG和两种未鉴定的极性脂质（L1和L3）的存在使BR5-29与其最接近的系统发育邻居区分开来。因此，该分离株代表了噬几丁质菌科中的一个新属和新种，为此提出名称汉京人参杆菌属（Ginsengibacter），新属，新种。模式菌株为BR5-29（=KACC 19446=LMG 30462）。因此，我们预测这种新型菌株可能对未来的研究分析（目标基因克隆）和Rg3的大规模生产有用。
