Lee On On, Lau Stanley C K, Tsoi Mandy M Y, Li Xiancui, Plakhotnikova Ioulia, Dobretsov Sergey, Wu Madeline C S, Wong Po-Keung, Qian Pei-Yuan
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, People's Republic of China.
Division of Environmental Science and Engineering, The National University of Singapore, Singapore.
Int J Syst Evol Microbiol. 2006 Aug;56(Pt 8):1795-1799. doi: 10.1099/ijs.0.64345-0.
A yellow-pigmented, Gram-negative, rod-shaped, strictly aerobic bacterium (strain UST050418-085(T)) was isolated from the surface of a marine sponge, Myxilla incrustans, at Friday Harbor, WA, USA. The DNA G+C content of this strain was 34.6 mol%. The predominant fatty acids were i15 : 0, a15 : 0, i15 : 1, i16 : 0, i17 : 0 3-OH, 17 : 0 2-OH and summed feature 3, comprising i15 : 0 2-OH and/or 16 : 1omega7c (altogether representing 69.0 % of the total fatty acids). MK-6 was the only respiratory quinone detected. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of UST050418-085(T) were members of the genus Gillisia, with sequence similarities of 93.2-96.6 %. Strain UST050418-085(T) differed from its closest relatives by 11 to 18 phenotypic traits. Molecular evidence and phenotypic characteristics suggest that strain UST050418-085(T) represents a novel species within the genus Gillisia. The name Gillisia myxillae sp. nov. is proposed, with UST050418-085(T) (=JCM 13564(T)=NRRL B-41416(T)) [corrected] as the type strain.
从美国华盛顿州星期五港的一种海洋海绵——硬壳粘管海绵（Myxilla incrustans）表面分离出一株黄色色素沉着、革兰氏阴性、杆状、严格需氧的细菌（菌株UST050418-085(T)）。该菌株的DNA G+C含量为34.6 mol%。主要脂肪酸为i15 : 0、a15 : 0、i15 : 1、i16 : 0、i17 : 0 3-OH、17 : 0 2-OH以及特征性脂肪酸总和3，后者由i15 : 0 2-OH和/或16 : 1ω7c组成（总共占总脂肪酸的69.0%）。检测到的唯一呼吸醌为MK-6。基于16S rRNA基因序列的系统发育分析表明，UST050418-085(T)最接近的亲缘种是吉利氏菌属（Gillisia）的成员，序列相似性为93.2 - 96.6%。菌株UST050418-085(T)与其最接近的亲缘种在11至18个表型特征上存在差异。分子证据和表型特征表明，菌株UST050418-085(T)代表吉利氏菌属内的一个新物种。提议将其命名为粘管海绵吉利氏菌（Gillisia myxillae）新种，以UST050418-085(T)（=JCM 13564(T)=NRRL B-41416(T)）[已校正]作为模式菌株。
