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三种组织化学染色方法用于检测肝脏铜储存异常的白鲈(美洲条纹鲈)中铜的比较

Comparison of three histochemical staining methods for the detection of copper in white perch (Morone americana) with abnormal hepatic copper storage.

作者信息

Bunton T E

机构信息

Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

出版信息

J Comp Pathol. 1990 Jan;102(1):25-31. doi: 10.1016/s0021-9975(08)80004-7.

DOI:10.1016/s0021-9975(08)80004-7
PMID:1690227
Abstract

A comparison of orcein, rubeanic acid and rhodanine histochemical stains in serial sections of liver from white perch (Morone americana) with abnormal hepatic copper storage is described. Staining reactions correlated well with hepatic copper concentrations although the threshold was the most problematic for rhodanine and rubeanic acid. Orcein was positive at a lower concentration than the two other stains. Hepatocellular granules and macrophage centres increased in size and number with copper concentration and could be related to increased lysosomes, as described for other species.

摘要

描述了对美洲河鲈(Morone americana)肝脏连续切片中orcein、硫氰酸和罗丹宁组织化学染色的比较,该肝脏存在异常肝铜蓄积。染色反应与肝铜浓度密切相关,尽管罗丹宁和硫氰酸的阈值最成问题。orcein在比其他两种染色更低的浓度时呈阳性。肝细胞颗粒和巨噬细胞中心的大小和数量随铜浓度增加而增加,并且可能与溶酶体增加有关,正如对其他物种所描述的那样。

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