Kumaratilake J S, Howell J M
Res Vet Sci. 1987 Jan;42(1):73-81.
Six Merino sheep were dosed orally with a 0.2 per cent solution of copper sulphate, six others were undosed controls. Liver biopsies were obtained and stained for copper by the p-dimethylaminobenzylidene rhodanine (DMABR), rubeanic acid (RA) and ferricyanide (FCN) methods for examination by light microscopy. The initial and most marked accumulations of copper were found within the hepatocytes of the centrilobular zones. Increased copper loading resulted in copper deposition extending through the midlobular to the periportal zones. The deposition of copper was unequal between hepatocytes and with increasing copper loading isolated hepatocytes became packed with copper containing granules. Copper appeared within Kupffer cells and macrophages of portal triads. The first Kupffer cells to be positively stained and the greatest number of such cells were adjacent to the central veins. Accumulation of copper was demonstrated with hepatocytes at copper concentrations equivalent to 44.3 micrograms copper g-1 liver wet weight. The FCN method provided a more satisfactory demonstration of intracellular copper than the RA technique and the latter was better than the DMABR method. However, the DMABR technique provided the clearest morphological details.
给六只美利奴绵羊口服0.2%的硫酸铜溶液，另外六只为未给药的对照。获取肝脏活检组织，并用对二甲基氨基苄叉罗丹宁（DMABR）、赤藓酸（RA）和铁氰化物（FCN）方法对铜进行染色，以便通过光学显微镜检查。最初且最显著的铜蓄积见于小叶中心区的肝细胞内。铜负荷增加导致铜沉积从小叶中部延伸至门周区。肝细胞之间的铜沉积不均，随着铜负荷增加，单个肝细胞充满含铜颗粒。在门三联的库普弗细胞和巨噬细胞内出现了铜。最早被阳性染色的库普弗细胞以及此类细胞的最大数量位于中央静脉附近。当肝脏湿重中铜浓度相当于44.3微克铜/克时，肝细胞出现铜蓄积。与RA技术相比，FCN方法对细胞内铜的显示更令人满意，而RA技术又优于DMABR方法。然而，DMABR技术提供了最清晰的形态学细节。
