Thornburg L P, Beissenherz M, Dolan M, Raisbeck M F
Vet Pathol. 1985 Jul;22(4):327-32. doi: 10.1177/030098588502200405.
Three different histochemical methods for copper detection were compared. Atomic absorption analysis was used to substantiate the tissue stains. There was good correlation between rhodanine staining and rubeanic acid-stained tissue sections. The orcein reaction for copper-associated protein did not consistently correlate with the methods demonstrating copper. Prolonged staining (72 hours) with rubeanic acid more consistently and clearly detected increased copper in canine livers than did staining with rhodanine. Seventy-two hour staining with rubeanic acid is the method of choice for histochemical detection of copper in canine liver.
比较了三种不同的铜检测组织化学方法。采用原子吸收分析法来证实组织染色结果。罗丹宁染色与红氨酸染色的组织切片之间存在良好的相关性。用于检测铜相关蛋白的orcein反应与其他显示铜的方法之间并无一致的相关性。与罗丹宁染色相比，用红氨酸进行长时间染色（72小时）能更一致且清晰地检测出犬肝脏中铜含量的增加。72小时红氨酸染色是组织化学检测犬肝脏中铜的首选方法。
