Egberink H F, Ederveen J, Montelaro R C, Pedersen N C, Horzinek M C, Koolen M J
Department of Virology, Veterinary Faculty, State University, Utrecht, The Netherlands.
J Gen Virol. 1990 Mar;71 ( Pt 3):739-43. doi: 10.1099/0022-1317-71-3-739.
Feline immunodeficiency virus (FIV) grown in cat lymphocyte and thymocyte cultures was labelled with L-[35S]methionine or [3H]glucosamine and virus-coded proteins were identified using immunoprecipitation. Polypeptides with apparent Mr values of 15K, 24K, 43K, 50K, 120K and 160K were detected. An additional polypeptide of 10K was detected by Western blot analysis. The two highest Mr species sometimes appeared as one band, of which only the 120K polypeptide was glycosylated. In the presence of tunicamycin gp120 was no longer detectable and a non-glycosylated precursor of 75K was found instead. Pulse-chase experiments suggested that the smaller polypeptides p24 and p15 are cleavage products of both p160 and p50. Western blot analysis using a rabbit serum directed against p26 of equine infectious anaemia virus (EIAV) and an anti-EIAV horse serum from a field case of infection revealed a cross-reactivity with p24 of FIV. Cat sera collected late after experimental FIV infection recognized p26 of EIAV, indicating a reciprocal cross-reactivity.
在猫淋巴细胞和胸腺细胞培养物中培养的猫免疫缺陷病毒（FIV）用L-[35S]甲硫氨酸或[3H]葡糖胺进行标记，并用免疫沉淀法鉴定病毒编码的蛋白质。检测到表观分子量值为15K、24K、43K、50K、120K和160K的多肽。通过蛋白质印迹分析检测到一条额外的10K多肽。两种分子量最高的蛋白有时表现为一条带，其中只有120K多肽被糖基化。在衣霉素存在的情况下，不再能检测到gp120，而是发现了一种75K的非糖基化前体。脉冲追踪实验表明，较小的多肽p24和p15是p160和p50的裂解产物。使用针对马传染性贫血病毒（EIAV）p26的兔血清和来自野外感染病例的抗EIAV马血清进行蛋白质印迹分析，结果显示与FIV的p24有交叉反应性。实验性FIV感染后晚期采集的猫血清可识别EIAV的p26，表明存在相互交叉反应性。
