Buzády Andrea, Erostyák János, Paál Gábor
Department of Experimental Physics, University of Pécs, H-7624 Pécs, Ifjúság u. 6., Hungary.
J Biochem Biophys Methods. 2006 Nov 30;69(1-2):207-14. doi: 10.1016/j.jbbm.2006.05.009. Epub 2006 Jun 2.
The underground water system of Mecsek Hill, Hungary was studied using spectrofluorometry. A quantitative dye-tracing technique was used to map the connections between ground water recharge points and the springs and wells. Excitation-emission matrices were used to establish optimal spectroscopic parameters for spectral measurements. Only an EEM gives full spectrofluorimetric information about the sample and makes possible the determination of the optimal wavelength parameters. The uranine concentration was determined by measuring the synchronous excitation spectra of water samples. It was found that under the 4 microg/L uranine concentration, no special chemical treatment was necessary if the pH values remained between 7 and 8. However, above this concentration, the solution should be diluted to avoid spectral distortion from the increasing absorbance of the sample. Long-term concentration measurements were conducted based on spectrofluorometry.
利用荧光分光光度法对匈牙利迈切克山的地下水系统进行了研究。采用定量染料示踪技术绘制地下水补给点与泉水及水井之间的联系。利用激发-发射矩阵确定光谱测量的最佳光谱参数。只有激发-发射矩阵能提供有关样品的完整荧光光谱信息，并使确定最佳波长参数成为可能。通过测量水样的同步激发光谱来测定尿苷浓度。结果发现，在尿苷浓度为4微克/升以下时，如果pH值保持在7至8之间，则无需进行特殊化学处理。然而，高于此浓度时，应稀释溶液以避免样品吸光度增加导致光谱失真。基于荧光分光光度法进行了长期浓度测量。
