Chen Hongqi, Yu Hupeng, Zhou Yunyou, Wang Lun
College of Chemistry and Material Science, Anhui Key Laboratory of Functional, Molecular Solids, Anhui Normal University, Wuhu 241000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2007 Jul;67(3-4):683-6. doi: 10.1016/j.saa.2006.07.057. Epub 2006 Sep 3.
A simple and sensitive fluorescent quenching method for the determination of trace hydrogen peroxide (H(2)O(2)) has been proposed to determine hydrogen peroxide in rain water sample. The method is based on the reaction of H(2)O(2) with 3,3'-diethyloxadicarbocyanine iodide (DI) to form a compound which has no fluorescence in acetate buffer solution (pH 3.09). The maximum emission wavelength of the system is located at 604 nm with excitation at 570 nm. Under the optimal conditions, the calibration graph was obtained between the quenched fluorescence intensity and hydrogen peroxide concentration in the range of 5.0 x 10(-7) to 9.0 x 10(-4) mol L(-1). The proposed method was applied to determine H(2)O(2) in rain water samples, and the result was satisfactory. The mechanism involved in the reaction was also studied.
提出了一种简单灵敏的荧光猝灭法测定痕量过氧化氢（H₂O₂），用于测定雨水样品中的过氧化氢。该方法基于H₂O₂与3,3'-二乙基氧杂二碳菁碘化物（DI）反应形成一种在醋酸盐缓冲溶液（pH 3.09）中无荧光的化合物。该体系的最大发射波长位于604 nm，激发波长为570 nm。在最佳条件下，得到了猝灭荧光强度与过氧化氢浓度在5.0×10⁻⁷至9.0×10⁻⁴ mol L⁻¹范围内的校准曲线。将该方法应用于雨水样品中H₂O₂的测定，结果令人满意。还研究了反应涉及的机理。
