Henrickson K J, Portner A
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38101-0318.
Vaccine. 1990 Feb;8(1):75-80. doi: 10.1016/0264-410x(90)90182-l.
The antibody response in children to known epitopes on the HN of human parainfluenza virus type 3 was investigated. Children's sera with Haemagglutination-Inhibition titres between 1/480 to 1/1280 were used. When tested by ELISA, this high-titre serum from each of five children blocked 7 of 17 specific anti-HN murine monoclonal antibodies by greater than 75% at 1 micrograms well-1 of antigen. However, four monoclonal antibodies were blocked less than or equal to 30%, while six were partially blocked between 50% and 75%. Antigen concentrations of 0.5, 1.5 and 2.0 micrograms well-1 did not substantially change this pattern. Comparison of our results with published antigenic maps indicated that antigenic site A on the HN protein was the site with the most significant antibody representation in the children's sera. These findings suggest that antigenic maps deduced using monoclonal antibodies need to be carefully interpreted before they are used in vaccine development. Murine monoclonal antibodies may not fully represent either qualitatively or quantitatively important antibody components of the human or murine immune response to human PIV-3 HN.
对3型人副流感病毒血凝素-神经氨酸酶（HN）上已知表位的儿童抗体反应进行了研究。使用血凝抑制效价在1/480至1/1280之间的儿童血清。通过酶联免疫吸附测定（ELISA）检测时，来自五名儿童的这种高滴度血清在抗原浓度为1微克/孔时，能使17种抗HN鼠单克隆抗体中的7种被阻断超过75%。然而，有4种单克隆抗体被阻断程度小于或等于30%，6种单克隆抗体被部分阻断，阻断程度在50%至75%之间。抗原浓度为0.5微克/孔、1.5微克/孔和2.0微克/孔时，这一模式没有实质性变化。将我们的结果与已发表的抗原图谱进行比较表明，HN蛋白上的抗原位点A是儿童血清中抗体呈现最为显著的位点。这些发现表明，在用于疫苗开发之前，对使用单克隆抗体推导的抗原图谱需要谨慎解读。鼠单克隆抗体可能在质量或数量上都无法完全代表人类或小鼠对人PIV-3 HN免疫反应中重要的抗体成分。
