Konttinen Y T, Kinnunen E, Kemppinen P, Juntunen J, Koskenvuo M
Fourth Department of Medicine, University Central Hospital, Helsinki, Finland.
J Neuroimmunol. 1990 Apr;27(1):1-8. doi: 10.1016/0165-5728(90)90130-f.
Phytohemagglutinin-induced lymphocyte activation sequence was studied in monozygotic and dizygotic discordant multiple sclerosis (MS) twin pairs in a quiescent disease phase. The study group included all available 11 pairs listed in a nation-wide twin register. Lymphocyte activation markers, DNA synthesis and gamma-interferon secretion were studied using avidin-biotin-peroxidase complex (ABC) stainings, [3H]thymidine incorporation, and a solid-phase double-antibody immunoradiometric assay (IRMA), respectively. The level and kinetics of interleukin-2 receptor expression, DNA synthesis, gamma-interferon secretion, and major histocompatibility complex (MHC) locus II antigen expression were similar (Wilcoxon's test for paired samples) in both the diseased and healthy monozygotic and dizygotic twins. Our results suggest that the cell-mediated immune system may not be primarily at fault, but rather that both MS itself and its exacerbations are caused by an unknown triggering stimulus facing a properly functioning immune system.
在疾病静止期的单卵和双卵不一致性多发性硬化症（MS）双胞胎对中，研究了植物血凝素诱导的淋巴细胞激活序列。研究组包括全国双胞胎登记册中列出的所有11对可用双胞胎。分别使用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）染色、[3H]胸腺嘧啶核苷掺入法和固相双抗体免疫放射测定法（IRMA）研究淋巴细胞激活标志物、DNA合成和γ-干扰素分泌。在患病和健康的单卵及双卵双胞胎中，白细胞介素-2受体表达、DNA合成、γ-干扰素分泌和主要组织相容性复合体（MHC）II类抗原表达的水平及动力学相似（配对样本的Wilcoxon检验）。我们的结果表明，细胞介导的免疫系统可能并非主要问题所在，而是MS本身及其病情加重是由面对正常运作的免疫系统的未知触发刺激所导致的。
