Darr D, McCormack K M, Manning T, Dunston S, Winston D C, Schulte B A, Buller T, Pinnell S R
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
J Cutan Pathol. 1990 Feb;17(1):9-15. doi: 10.1111/j.1600-0560.1990.tb01671.x.
Angiogenesis is necessary for normal growth, wound healing, and plays a key role in many pathologic processes. A variety of endothelial markers have been used to investigate angiogenesis. Unfortunately, excellent markers for vascular endothelium in human tissues exhibit little or no staining of endothelia in tissues of other animal species, including the pig. We are interested in the hairless Yucatan strain of mini-pig as an animal model for studying cutaneous wound healing because its skin is histologically and functionally very similar to that of man. Hoping to find a specific marker to identify vascular endothelium in the mini-pig, we therefore screened a battery of 11 different lectin-horseradish peroxidase conjugates. Based on specificity and staining intensity, Dolichos biflorus agglutinin (DBA) was chosen from this battery to investigate vascular changes in the healing of cutaneous wounds in the mini-pig. When compared with routine histologic sections stained with hematoxylin and eosin, blood vessels were much easier to identify in sections stained histochemically with DBA. Lectin histochemistry was particularly useful in investigations of early events in angiogenesis during wound healing when newly derived capillary buds and minute blood vessels were obscured in normal histologic sections by an inflammatory cell infiltrate associated with the healing wound. Ultrastructural lectin cytochemistry revealed staining along the luminal surface and the basolateral plasmalemma of endothelial cells. Histochemical staining with DBA promises to provide a useful method for further investigation of angiogenesis and other vascular phenomena in a variety of normal and pathologic processes using the hairless Yucatan strain of mini-pig as the animal model.
血管生成对于正常生长、伤口愈合是必需的，并且在许多病理过程中起关键作用。多种内皮标志物已被用于研究血管生成。不幸的是，用于人类组织中血管内皮的优秀标志物在包括猪在内的其他动物物种的组织中对内皮几乎没有或没有染色。我们对无毛尤卡坦小型猪品系作为研究皮肤伤口愈合的动物模型感兴趣，因为其皮肤在组织学和功能上与人类皮肤非常相似。因此，希望找到一种特异性标志物来识别小型猪中的血管内皮，我们筛选了一系列11种不同的凝集素-辣根过氧化物酶缀合物。基于特异性和染色强度，从该系列中选择了双花扁豆凝集素（DBA）来研究小型猪皮肤伤口愈合过程中的血管变化。与用苏木精和伊红染色的常规组织学切片相比，用DBA进行组织化学染色的切片中血管更容易识别。凝集素组织化学在研究伤口愈合过程中血管生成的早期事件时特别有用，此时新形成的毛细血管芽和微小血管在正常组织学切片中被与愈合伤口相关的炎性细胞浸润所掩盖。超微结构凝集素细胞化学显示在内皮细胞的腔表面和基底外侧质膜上有染色。用DBA进行组织化学染色有望为以无毛尤卡坦小型猪品系作为动物模型，进一步研究各种正常和病理过程中的血管生成及其他血管现象提供一种有用的方法。
