Williams R J, Robertson D, Davies A J
Section of Biology and Surgery, Chester Beatty Laboratories, London, UK.
Histochem J. 1989 May;21(5):271-8. doi: 10.1007/BF01757179.
The purpose of this study was to determine whether the plant lectin, Dolichos biflorus agglutinin (DBA), can be used to recognize capillary endothelial cells and their processes during angiogenesis. By means of a peroxidase conjugate of DBA, blood vessels were visualized in whole mounts and ultrathin sections of mouse omentum. A part of this mesentery normally comprises an avascular membrane that is approximately 30 microns in thickness. Changes in the vascular plexus bordering this membrane were induced by intraperitoneal injection of irradiated Landschutz cells. Vascular endothelial cells were precisely and intensely stained, and vasculogenic processes were reliably distinguished from those of other cells. This technique permitted observation of the structure and distribution of capillary sprouts, and their relationship to each other and to pre-existing blood vessels. It was discovered that filiform projections extend from sprout apices. These projections may fuse allowing adjacent sprouts to form a new capillary loop.
本研究的目的是确定植物凝集素双花扁豆凝集素（DBA）是否可用于识别血管生成过程中的毛细血管内皮细胞及其突起。通过DBA的过氧化物酶偶联物，在小鼠网膜的整装标本和超薄切片中观察血管。该肠系膜的一部分通常由厚度约为30微米的无血管膜组成。通过腹腔注射经辐照的兰氏细胞诱导毗邻该膜的血管丛发生变化。血管内皮细胞被精确且强烈地染色，并且血管生成过程能够可靠地与其他细胞的过程区分开来。该技术允许观察毛细血管芽的结构和分布，以及它们彼此之间以及与先前存在的血管的关系。研究发现丝状突起从芽尖伸出。这些突起可能融合，使相邻的芽形成新的毛细血管环。
