Hua Dongliang, Ma Cuiqing, Song Lifu, Lin Shan, Zhang Zhaobin, Deng Zixin, Xu Ping
State Key Laboratory of Microbial Technology, Shandong University, Jinan, PR China.
Appl Microbiol Biotechnol. 2007 Mar;74(4):783-90. doi: 10.1007/s00253-006-0735-5. Epub 2006 Nov 24.
High vanillin productivity was achieved in the batch biotransformation of ferulic acid by Streptomyces sp. strain V-1. Due to the toxicity of vanillin and the product inhibition, fed-batch biotransformation with high concentration of ferulic acid was unsuccessful. To solve this problem and improve the vanillin yield, a biotransformation strategy using adsorbent resin was investigated. Several macroporous adsorbent resins were chosen to adsorb vanillin in situ during the bioconversion. Resin DM11 was found to be the best, which adsorbed the most vanillin and the least ferulic acid. When 8% resin DM11 (wet w/v) was added to the biotransformation system, 45 g l(-1) ferulic acid could be added continually and 19.2 g l(-1) vanillin was obtained within 55 h, which was the highest vanillin yield by bioconversion until now. This yield was remarkable for exceeding the crystallization concentration of vanillin and therefore had far-reaching consequence in its downstream processing.
链霉菌V-1菌株在阿魏酸的分批生物转化中实现了高香草醛产量。由于香草醛的毒性和产物抑制作用，高浓度阿魏酸的补料分批生物转化未成功。为了解决这个问题并提高香草醛产量，研究了一种使用吸附树脂的生物转化策略。选择了几种大孔吸附树脂在生物转化过程中原位吸附香草醛。发现树脂DM11是最好的，它吸附的香草醛最多，阿魏酸最少。当向生物转化系统中添加8%（湿重/体积）的树脂DM11时，可以连续添加45 g l(-1)阿魏酸，并在55小时内获得19.2 g l(-1)香草醛，这是迄今为止通过生物转化获得的最高香草醛产量。该产量非常显著，因为它超过了香草醛的结晶浓度，因此对其下游加工具有深远影响。
