Norris Tracy B, Castenholz Richard W
Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403, USA.
FEMS Microbiol Ecol. 2006 Sep;57(3):470-83. doi: 10.1111/j.1574-6941.2006.00134.x.
Molecular and culture based methods were used to survey endolithic, photosynthetic communities from hot spring-formed travertine rocks of various ages, ranging from<10 to greater than 300,000 years. Much of this travertine contained a 1-3-mm-thick greenish band composed mainly of cyanobacteria 1-5 mm below the rock surface. The travertine rocks experienced desiccation in summer and freezing in winter. A total of 83 environmental 16S rRNA gene sequences were obtained from clone libraries and denaturing gradient gel electrophoresis. Small subunit rRNA gene sequences and cell morphology were determined for 36 cyanobacterial culture isolates from these samples. Phylogenetic analysis showed that the 16S rRNA gene sequences fell into 15 distinct clusters, including several novel lineages of cyanobacteria.
采用分子和培养方法，对不同年代（距今不到10年至超过30万年）温泉形成的钙华岩中的内生光合群落进行了调查。这些钙华岩大部分含有一条1 - 3毫米厚的绿色带，主要由位于岩石表面以下1 - 5毫米处的蓝细菌组成。钙华岩在夏季经历干燥，冬季经历冰冻。从克隆文库和变性梯度凝胶电泳中总共获得了83个环境16S rRNA基因序列。对这些样品中36株蓝细菌培养分离株进行了小亚基rRNA基因序列和细胞形态测定。系统发育分析表明，16S rRNA基因序列分为15个不同的簇，包括几个蓝细菌新谱系。
