白细胞介素 1A 和白细胞介素 1B 基因多态性与葡萄牙人群种植体周围炎的相关性：一项初步研究。
Association between IL-1A and IL-1B gene polymorphisms with peri-implantitis in a Portuguese population-a pilot study.
机构信息
Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Instituto Universitário Egas Moniz, Almada, Portugal.
Periodontology Department, Instituto Universitário Egas Moniz, Almada, Portugal.
出版信息
PeerJ. 2022 Jul 14;10:e13729. doi: 10.7717/peerj.13729. eCollection 2022.
BACKGROUND
Scientific evidence indicates that biological complications in dental implants tend to be concentrated in a subset of individuals, which seems to imply that the host response may play a determining role in implant success. Over the last few decades, several polymorphisms have been studied. Polymorphisms in the interleukin (IL) 1 gene cluster have been associated with periodontitis. There are some similar features in the sequence of immunopathological events in peri-implant and periodontal infections. We aimed to investigate if individuals carrying the genetic single nucleotide polymorphism (SNP) in the IL-1A (rs1800587) and IL-1B (rs1143634) genes are more susceptible to develop peri-implantitis.
METHODS
A cross-sectional analytic pilot study was conducted in 20 Caucasian Portuguese subjects divided into two groups: 10 subjects with peri-implantitis and 10 subjects with peri-implant health (control group). Samples containing cells from the buccal mucosa were stored at -20 °C and later submitted to the DNA extraction process. Genetic analysis was performed using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. Data were analyzed by using descriptive and inferential statistical methodologies.
RESULTS
For the IL-1A (-889) gene polymorphism, it was observed that the mutated allele was present in a higher percentage in the peri-implantitis group compared to the control group (30% vs 15% respectively, Fisher's exact test, = 0.45). For the IL-1B (+3954) gene polymorphism, it was also observed that the altered allele was present in a higher percentage in the disease group compared to the control group (35% vs 10% respectively, Fisher's exact test, = 0.13). The positive genotype (at least one allele with nucleotide sequence changed in both genes) was detected in six patients, five belonging to the disease group and one to the health group.
CONCLUSIONS
Regarding IL-1 gene polymorphisms, there was no statistically significant difference between the health and disease group, however a trend should be highlighted, showing a potential link between the IL-1 genotype and peri-implantitis. More studies are needed to clarify the role of genetic polymorphisms in the development of peri-implantitis.
背景
科学证据表明，牙科种植体的生物学并发症往往集中在一部分人群中，这似乎表明宿主反应可能在种植体成功中起决定性作用。在过去的几十年中，已经研究了几种多态性。白细胞介素（IL）1 基因簇中的多态性与牙周炎有关。在种植体周围和牙周感染的免疫病理事件序列中存在一些相似的特征。我们旨在研究携带白细胞介素 1A（rs1800587）和白细胞介素 1B（rs1143634）基因中遗传单核苷酸多态性（SNP）的个体是否更容易发生种植体周围炎。
方法
在 20 名葡萄牙白种人受试者中进行了一项横断面分析性试点研究，将他们分为两组：10 名患有种植体周围炎的受试者和 10 名种植体周围健康的受试者（对照组）。含有颊黏膜细胞的样本储存在-20°C 下，然后进行 DNA 提取过程。使用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析进行基因分析。使用描述性和推断性统计方法分析数据。
结果
对于 IL-1A（-889）基因多态性，观察到在种植体周围炎组中，突变等位基因的存在百分比高于对照组（分别为 30%和 15%，Fisher 确切检验，=0.45）。对于 IL-1B（+3954）基因多态性，也观察到在疾病组中，改变的等位基因的存在百分比高于对照组（分别为 35%和 10%，Fisher 确切检验，=0.13）。在六名患者中检测到阳性基因型（两个基因中至少有一个核苷酸序列改变的等位基因），其中五名属于疾病组，一名属于健康组。
结论
关于 IL-1 基因多态性，健康组和疾病组之间没有统计学上的显著差异，但应强调一个趋势，表明 IL-1 基因型与种植体周围炎之间存在潜在联系。需要进一步研究来阐明遗传多态性在种植体周围炎发展中的作用。
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