Marano R J, Rakoczy P E
Department of Molecular Ophthalmology, Lions Eye Institute, Nedlands, Western Australia, Australia.
Biotech Histochem. 2006 Mar-Jun;81(2-3):59-62. doi: 10.1080/10520290600799131.
Laser photocoagulation induced choroidal neovascularization currently is the most effective model available for the study of this disease in terms of efficacy of new drugs and therapies. Previously, evaluating the extent of choroidal neovascularization using this model was time-consuming and required the use of experienced personnel. We describe a new method for simple and rapid evaluation of laser induced choroidal neovascularization using densitometry. Fluorescein angiograms of a laser photocoagulated rat eye were scanned into a computer. Densitometry software subsequently was used to calculate the severity of the laser lesions. The densitometry method proved effective for calculating the extent of laser induced choroidal neovascularization. In addition, this method was more rapid than visual evaluations and less likely to produce errors.
就新药和治疗方法的疗效而言，激光光凝诱导脉络膜新生血管形成是目前可用于研究该疾病的最有效模型。以前，使用该模型评估脉络膜新生血管形成的程度既耗时又需要经验丰富的人员。我们描述了一种使用光密度测定法简单快速评估激光诱导脉络膜新生血管形成的新方法。将激光光凝大鼠眼睛的荧光血管造影扫描到计算机中。随后使用光密度测定软件计算激光损伤的严重程度。光密度测定法被证明对计算激光诱导脉络膜新生血管形成的程度有效。此外，该方法比视觉评估更快，产生误差的可能性更小。
