Mayer B J, Hanafusa H
Rockefeller University, New York, NY 10021.
Proc Natl Acad Sci U S A. 1990 Apr;87(7):2638-42. doi: 10.1073/pnas.87.7.2638.
An antiserum specific for P47gag-crk, the oncogene product of avian sarcoma virus CT10, was used to investigate possible crk-binding proteins. In in vitro kinase assays, four proteins were phosphorylated in anti-crk immunoprecipitates. Tyrosine, serine, and threonine residues were phosphorylated. A number of tyrosine-phosphorylated proteins were identified in anti-crk immunoprecipitates of 32P-labeled cells, including the three major phosphotyrosine-containing proteins of CT10-infected cells. These three proteins also bound to bacterially synthesized crk protein. These results suggest that the crk transforming protein can stably associate with both endogenous kinases and cellular kinase substrates.
一种针对禽肉瘤病毒CT10的致癌基因产物P47gag-crk的抗血清，被用于研究可能的crk结合蛋白。在体外激酶分析中，抗crk免疫沉淀物中有四种蛋白质发生了磷酸化。酪氨酸、丝氨酸和苏氨酸残基都被磷酸化。在32P标记细胞的抗crk免疫沉淀物中鉴定出了许多酪氨酸磷酸化蛋白，包括CT10感染细胞中的三种主要含磷酸酪氨酸的蛋白。这三种蛋白也与细菌合成的crk蛋白结合。这些结果表明，crk转化蛋白可以与内源性激酶和细胞激酶底物稳定结合。
