体外下颌髁突成骨分化过程中的基因表达
Gene expression during osteogenic differentiation in mandibular condyles in vitro.
作者信息
Strauss P G, Closs E I, Schmidt J, Erfle V
机构信息
GSF-Abteilung für Molekulare Zellpathologie, Neuherberg, Federal Republic of Germany.
出版信息
J Cell Biol. 1990 Apr;110(4):1369-78. doi: 10.1083/jcb.110.4.1369.
Abstract
The cartilagenous tissue of mandibular condyles of newborn mice contains progenitor cells as well as young and mature chondrogenic cells. During in vitro cultivation of the tissue, progenitor cells undergo osteogenic differentiation and form new bone (Silbermann, M., D. Lewinson, H. Gonen, M. A. Lizarbe, and K. von der Mark. 1983. Anat. Rec. 206:373-383). We have studied the expression of genes that typify osteogenic differentiation in mandibular condyles during in vitro cultivation. RNAs of the genes for collagen type I, osteonectin, alkaline phosphatase, and bone gla protein were sequentially expressed in progenitor cells and hypertrophic chondrocytes during culture. Osteopontin expression peaked in both the early and the late phase of the differentiation process. The data indicate a distinct sequence of expression of osteoblast-specific genes during osteogenic differentiation and new bone formation in mandibular condyles.
摘要
新生小鼠下颌髁突的软骨组织含有祖细胞以及年轻和成熟的软骨生成细胞。在该组织的体外培养过程中，祖细胞经历成骨分化并形成新骨（西尔伯曼，M.，D. 莱温森，H. 戈嫩，M. A. 利扎尔贝，和 K. 冯·德·马克。1983年。《解剖学记录》206:373 - 383）。我们研究了体外培养过程中下颌髁突中成骨分化典型基因的表达情况。在培养过程中，I型胶原、骨连接蛋白、碱性磷酸酶和骨钙蛋白基因的RNA在祖细胞和肥大软骨细胞中依次表达。骨桥蛋白的表达在分化过程的早期和晚期均达到峰值。数据表明，在下颌髁突的成骨分化和新骨形成过程中，成骨细胞特异性基因存在明显的表达顺序。
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本文引用的文献
1
An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex.一种改良的原位杂交方法，用于检测果蝇组织切片中的细胞 RNA，及其在定位同源异形 Antennapedia 基因复合物转录本中的应用。
EMBO J. 1983;2(4):617-23. doi: 10.1002/j.1460-2075.1983.tb01472.x.
2
[Electron microscopic observations on the cells in the resorption zone of epiphysial cartilage].[骨骺软骨吸收区细胞的电子显微镜观察]
Z Zellforsch Mikrosk Anat. 1961;54:1-38.
3
In vitro transformation of chondroprogenitor cells into osteoblasts and the formation of new membrane bone.软骨祖细胞在体外转化为成骨细胞并形成新的膜性骨。
Anat Rec. 1983 Aug;206(4):373-83. doi: 10.1002/ar.1092060404.
4
Structure of two human beta-actin-related processed genes one of which is located next to a simple repetitive sequence.两个人类β-肌动蛋白相关加工基因的结构，其中一个位于一个简单重复序列旁边。
EMBO J. 1983;2(5):757-61. doi: 10.1002/j.1460-2075.1983.tb01496.x.
5
The development of embryonic bone and cartilage in tissue culture.组织培养中胚胎骨与软骨的发育
Clin Orthop Relat Res. 1983 Apr(174):243-63.
6
Cloning and characterization of five overlapping cDNAs specific for the human pro alpha 1(I) collagen chain.人Ⅰ型前α1胶原链特异性的五个重叠cDNA的克隆与特性分析
Nucleic Acids Res. 1982 Oct 11;10(19):5925-34. doi: 10.1093/nar/10.19.5925.
7
Immunological studies on collagen type transition in chondrogenesis.软骨形成过程中胶原类型转变的免疫学研究。
Curr Top Dev Biol. 1980;14(Pt 2):199-225. doi: 10.1016/s0070-2153(08)60195-7.
8
Persistence of Epstein-Barr virus in the parotid gland.爱泼斯坦-巴尔病毒在腮腺中的持续存在。
J Virol. 1984 Sep;51(3):795-8. doi: 10.1128/JVI.51.3.795-798.1984.
9
An autoradiographic study of chondrocyte transformation into chondroclasts and osteocytes during bone formation in vitro.
Anat Rec. 1967 Aug;158(4):473-83. doi: 10.1002/ar.1091580410.
10
The biochemical importance of the hypertrophic cartilage cell area to enchondral bone formation.
J Bone Joint Surg Am. 1970 Jul;52(5):1025-32.
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