Derbyshire J B, Lesnick C E
University of Guelph, Ontario, Canada.
J Interferon Res. 1990 Feb;10(1):47-53. doi: 10.1089/jir.1990.10.47.
The aim of this study was to produce sustained activation of natural killer (NK) cells in newborn piglets by interferon (IFN) induction with polyinosinic:polycytidylic acid complexed with poly-L-lysine and carboxymethylcellulose [poly(ICLC)]. When a second dose of poly(ICLC) was given 24 or 48 h after the first dose, the piglets were found to be hyporeactive, and the levels of induced IFN were insufficient to activate NK cells in vivo or in vitro. A hyporeactive factor was demonstrated in the serum of piglets 24 and 48 h after they were treated with poly(ICLC), which inhibited IFN induction in vitro with poly(ICLC) but not with Newcastle disease virus. The IFN response of hyporeactive piglets was partially restored by the administration of prostaglandin (PG) F2 alpha at the same time as the second dose of poly(ICLC); piglets treated in this way at 3 days of age showed significantly higher lymphocytic NK activity at 5 days and 7 days than the activity in untreated piglets and in those given one or two doses of poly(ICLC) in the absence of PG F2 alpha. It was concluded that piglets develop hyporeactivity to IFN induction, but the IFN and NK cell activation responses to poly(ICLC) can be restored by treatment with PG.
本研究的目的是通过用与聚-L-赖氨酸和羧甲基纤维素复合的聚肌苷酸:聚胞苷酸[聚(ICLC)]诱导干扰素(IFN)，使新生仔猪的自然杀伤(NK)细胞持续活化。当在第一剂聚(ICLC)给药后24或48小时给予第二剂时，发现仔猪反应低下，诱导的IFN水平不足以在体内或体外激活NK细胞。在用聚(ICLC)处理仔猪后24和48小时，在其血清中证实了一种反应低下因子，该因子在体外抑制聚(ICLC)诱导的IFN，但不抑制新城疫病毒诱导的IFN。在给予第二剂聚(ICLC)的同时给予前列腺素(PG)F2α，可部分恢复反应低下仔猪的IFN反应；3日龄时以这种方式处理的仔猪在5日龄和7日龄时的淋巴细胞NK活性显著高于未处理仔猪以及在未给予PG F2α的情况下给予一剂或两剂聚(ICLC)的仔猪。得出的结论是，仔猪对IFN诱导产生反应低下，但用PG处理可恢复对聚(ICLC)的IFN和NK细胞活化反应。
