辣椒素对兔耳动脉对场刺激反应的双重作用。

Dual effects of capsaicin on responses of the rabbit ear artery to field stimulation.

作者信息

Moritoki H, Takase H, Tanioka A

机构信息

Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.

出版信息

Br J Pharmacol. 1990 Jan;99(1):152-6. doi: 10.1111/j.1476-5381.1990.tb14669.x.

DOI:10.1111/j.1476-5381.1990.tb14669.x

PMID:1691941

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1917500/

Abstract

The effects of capsaicin (Cap) on contractions of ring segments of rabbit ear artery induced by field stimulation were studied. 2. At low concentrations (0.3-3 microM) Cap caused transient enhancement and at higher concentrations (above 3 microM) inhibition of stimulation-induced contractions, without affecting noradrenaline (NA)-induced contractions. 3. In the continuous presence of high concentrations of Cap, rebound facilitation was observed after inhibition, and at this stage, Cap elicited less inhibition of the response. 4. Repeated application of Cap at 60 min intervals irreversibly desensitized the artery to the inhibitory effect of Cap. 5. Functional removal of the endothelium enhanced the facilitatory effect of low concentrations of Cap and attenuated its inhibitory effect. 6. Pretreatment with indomethacin abolished the facilitatory effect of Cap and enhanced its inhibitory effect, indicating that prostaglandins are involved in the action of Cap. The effect of indomethacin was more marked in preparations from which the endothelium had been removed. 7. Desensitization to substance P (SP) or substance K (SK), did not affect either the inhibitory or the facilitatory effect of Cap. 8. These results suggest that the dual effects of Cap on stimulation-induced contractions of rabbit ear artery may arise from the release of multiple mediators that act prejunctionally to modulate NA release. The stimulant effect seems to be mediated by prostanoids, while the inhibitory effect seems to be caused by a substance(s) that is not SP or SK. The possibility that the mediator is calcitonin gene-related peptide requires further study.

摘要

研究了辣椒素（Cap）对兔耳动脉环段场刺激诱导收缩的影响。2. 在低浓度（0.3 - 3 microM）时，Cap引起短暂增强，而在高浓度（高于3 microM）时抑制刺激诱导的收缩，且不影响去甲肾上腺素（NA）诱导的收缩。3. 在持续存在高浓度Cap的情况下，抑制后观察到反弹促进作用，在此阶段，Cap对反应的抑制作用较小。4. 每隔60分钟重复应用Cap会使动脉对Cap的抑制作用不可逆地脱敏。5. 功能性去除内皮增强了低浓度Cap的促进作用并减弱了其抑制作用。6. 用吲哚美辛预处理消除了Cap的促进作用并增强了其抑制作用，表明前列腺素参与了Cap的作用。吲哚美辛的作用在去除内皮的制剂中更明显。7. 对P物质（SP）或K物质（SK）脱敏并不影响Cap的抑制或促进作用。8. 这些结果表明，Cap对兔耳动脉刺激诱导收缩的双重作用可能源于多种介质的释放，这些介质在节前起作用以调节NA释放。刺激作用似乎由前列腺素介导，而抑制作用似乎由非SP或SK的物质引起。介质是否为降钙素基因相关肽的可能性需要进一步研究。