Latza U, Niedobitek G, Schwarting R, Nekarda H, Stein H
Department of Pathology, Klinikum Steglitz, Free University Berlin, West Germany.
J Clin Pathol. 1990 Mar;43(3):213-9. doi: 10.1136/jcp.43.3.213.
A new monoclonal antibody, Ber-EP4, directed against a partially formol resistant epitope on the protein moiety of two 34 kilodalton and 39 kilodalton glycopolypeptides on human epithelial cells is described. Immunostaining of a wide range of normal and neoplastic human tissues and cell lines showed that all carcinomas and all non-neoplastic epithelial cells, except hepatocytes, parietal cells, and apical cell layers in squamous epithelia, homogeneously expressed Ber-EP4 antigen. As Ber-EP4 does not detect any normal or neoplastic non-epithelial cells, this antibody might prove valuable for the differentiation of the following (i) non-epithelial tumours from undifferentiated carcinomas; (ii) hepatocytes from bile duct cells in certain liver diseases; (iii) mesothelial cells from carcinoma cells in lung biopsy specimens; and (iv) reactive mesothelial cells from carcinoma cells in smears of serous effusions.
描述了一种新的单克隆抗体Ber-EP4，它针对人上皮细胞上两种34千道尔顿和39千道尔顿糖多肽蛋白质部分上的部分甲醛抗性表位。对多种正常和肿瘤性人类组织及细胞系进行免疫染色显示，除肝细胞、壁细胞和鳞状上皮的表层细胞外，所有癌组织和所有非肿瘤性上皮细胞均均匀表达Ber-EP4抗原。由于Ber-EP4不能检测到任何正常或肿瘤性非上皮细胞，该抗体可能在以下方面的鉴别中具有价值：（i）未分化癌与非上皮肿瘤；（ii）某些肝脏疾病中胆管细胞与肝细胞；（iii）肺活检标本中癌细胞与间皮细胞；（iv）浆液性积液涂片中癌细胞与反应性间皮细胞。
