Lauder I, Holland D, Mason D Y, Gowland G, Cunliffe W J
Histopathology. 1984 Mar;8(2):259-72. doi: 10.1111/j.1365-2559.1984.tb02340.x.
A total of 43 cases undifferentiated large cell tumours presenting in lymph nodes were stained by immunoperoxidase techniques using antibodies against keratin and a leucocyte-associated glycoprotein. In 26 cases diagnosed histologically as metastatic carcinoma, staining with the keratin antibody suggested a squamous cell origin in 11 cases. This was supported by microscopic observation of intracellular filaments in seven cases. In 15 patients in whom the original diagnosis was uncertain, a definite diagnosis was possible in all cases following immunoperoxidase staining with the two antibodies and most of these proved to be large cell lymphomas. In two cases a potentially major diagnostic error was detected. It is suggested that the staining of undifferentiated human neoplasms using combinations of antibodies reactive with epithelial and lymphoid cells should result in much greater diagnostic accuracy in the field of large cell tumours presenting in lymph nodes.
总共43例淋巴结出现的未分化大细胞肿瘤，采用抗角蛋白和白细胞相关糖蛋白抗体的免疫过氧化物酶技术进行染色。在26例经组织学诊断为转移性癌的病例中，用角蛋白抗体染色显示11例起源于鳞状细胞。7例通过细胞内细丝的显微镜观察得到了证实。在15例最初诊断不明确的患者中，用这两种抗体进行免疫过氧化物酶染色后，所有病例均能明确诊断，其中大多数被证明是大细胞淋巴瘤。检测到2例可能存在的重大诊断错误。有人认为，使用与上皮细胞和淋巴细胞反应的抗体组合对未分化人类肿瘤进行染色，在淋巴结出现的大细胞肿瘤领域应能大大提高诊断准确性。
