Zhao Chunxia, He Chaohong
Department of Chemical Engineering, Zhejiang University, Hangzhou, China.
J Sep Sci. 2006 Jul;29(11):1630-6. doi: 10.1002/jssc.200500464.
The medicinal plant Atractylodes macrocephala (Baizhu in Chinese) has been widely used in traditional Chinese medicine for energy and stomach complaints, treatment of dyspepsia and anorexia, anti-inflammation, anticancer and for increasing assimilation. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of two main bioactive components, namely, atractylon and atractylenolide III from A. macrocephala by using light petroleum (60-90 degrees C)-ethyl acetate-ethanol-water (4:1:4:1 v/v) as the two-phase solvent system in dual-mode elution. Compared with the separation using the normal-mode elution, the dual-mode HSCCC can be achieved with shorter elution time. Atractylenolide III (32.1 mg) at 99.0% purity and 319.6 mg atractylon at 97.8% purity could be obtained from 1000 mg crude sample in a single run. The recoveries of atractylenolide III and atractylon were 95.4 and 92.6%, respectively.
药用植物白术在中国传统医学中已被广泛用于治疗精力和胃部不适、消化不良和厌食症、抗炎、抗癌以及促进消化吸收。本文采用高速逆流色谱法（HSCCC），以正己烷（60 - 90℃）-乙酸乙酯-乙醇-水（4:1:4:1，v/v）为两相溶剂体系，在双模式洗脱条件下，从白术中制备分离和纯化了两个主要生物活性成分，即苍术酮和苍术内酯III。与常规洗脱模式相比，双模式HSCCC可在更短的洗脱时间内完成分离。单次运行可从1000 mg粗样品中获得纯度为99.0%的苍术内酯III（32.1 mg）和纯度为97.8%的苍术酮（319.6 mg）。苍术内酯III和苍术酮的回收率分别为95.4%和92.6%。
