产气荚膜梭菌CN3870血凝素与唾液酸酶之间的关系：生物活性蛋白的色谱表征
Relationship between hemagglutinin and sialidase from Clostridium perfringens CN3870: chromatographic characterization of the biologically active proteins.
作者信息
Rood J I, Wilkinson R G
出版信息
J Bacteriol. 1976 May;126(2):831-44. doi: 10.1128/jb.126.2.831-844.1976.
Abstract
Biochemical characterization of hemagglutinin and sialidase activities from Clostridium perfringens strain CN3870 revealed that this strain produced three sialidase enzymes that were separable to gel filtration, ion exchange chromatography, and polyacrylamide gel electrophoresis. The molecular weights of sialidase I, II, and III activities were 310,000 +/- 10,000, 105,000 +/- 4,000 and 64,000 +/- 2,000, respectively, the first figure being an approximate value only.
摘要
对产气荚膜梭菌CN3870菌株的血凝素和唾液酸酶活性进行生化特性分析发现，该菌株产生了三种唾液酸酶，可通过凝胶过滤、离子交换色谱和聚丙烯酰胺凝胶电泳分离。唾液酸酶I、II和III活性的分子量分别为310,000±10,000、105,000±4,000和64,000±2,000，第一个数值仅为近似值。
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