Passmore A J, Jarman S N, Swadling K M, Kawaguchi S, McMinn A, Nicol S
Institute of Antarctic and Southern Ocean Studies, University of Tasmania, Private Bag 77, Hobart, Tasmania, 7001, Australia.
Mar Biotechnol (NY). 2006 Nov-Dec;8(6):686-96. doi: 10.1007/s10126-005-6088-8. Epub 2006 Aug 23.
The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extraction, and identification of prey DNA from krill collected in the field. Group-specific polymerase chain reaction (PCR) was used to amplify diatom prey (Phylum: Bacillariophyta) and the results from DNA clone libraries were compared with microscopic diet analysis. DNA analysis was superior to microscopy for prey detection. However, differences in prey relative abundance estimates between the two techniques suggested some bias in the DNA-based estimates. Quantification showed that large amounts of prey DNA had been successfully preserved and extracted. Overall the results suggest that the application of DNA-based diet analysis to krill warrants further investigation, particularly for prey that are difficult to study using other methods.
人们已运用多种技术对南极磷虾（南极大磷虾）的饮食进行了研究，但目前的方法仍存在难以解决的问题。本研究探讨了一种利用DNA作为猎物生物标志物的替代方法。针对从野外采集的磷虾中猎物DNA的保存、提取和鉴定开发了相关方法。使用组特异性聚合酶链反应（PCR）来扩增硅藻猎物（门：硅藻门），并将DNA克隆文库的结果与显微镜下的饮食分析结果进行比较。在猎物检测方面，DNA分析优于显微镜检查。然而，两种技术在猎物相对丰度估计上的差异表明基于DNA的估计存在一些偏差。定量分析表明，大量的猎物DNA已成功保存并提取。总体而言，结果表明将基于DNA的饮食分析应用于磷虾值得进一步研究，特别是对于难以用其他方法研究的猎物。
