Feril Loreto B, Ogawa Ryohei, Tachibana Katsuro, Kondo Takashi
Department of Anatomy, Fukuoka University School of Medicine, Fukuoka, Japan.
Cancer Sci. 2006 Oct;97(10):1111-4. doi: 10.1111/j.1349-7006.2006.00286.x. Epub 2006 Aug 22.
Ultrasound-mediated gene transfection (sonotransfection) is a promising physical method for gene therapy, especially for cancer gene therapy. To investigate the optimal sonotransfection conditions and to determine whether the optimal transfection rate using sonotransfection is comparable to that of electrotransfection or liposome-mediated transfection, we sonicated different cancer cell lines (U937, HeLa, PC-3, Meth A and T-24) using a 1-MHz unfocused ultrasound at different intensities, pulse repetition frequencies and exposure times. The ideal ultrasound conditions were noted to be at 1.5 Watt/cm(2) pulsed at 0.5 Hz with a duty factor of 50%. The results showed that transfection rate increased with the number of pulses, and peaked between 10 and 15 pulses before it started to decline. Using such optimal conditions, we have shown that sonotransfection is superior to electrotransfection and liposome-mediated transfection at the fixed conditions used in the present study. These findings suggest that sonotransfection could be a better alternative to other non-viral methods (e.g. electroporation and liposome-mediated transfection) of gene transfection, particularly in cancer gene therapy.
超声介导的基因转染（声孔效应转染）是一种很有前景的基因治疗物理方法，尤其适用于癌症基因治疗。为了研究最佳声孔效应转染条件，并确定使用声孔效应转染的最佳转染率是否与电穿孔转染或脂质体介导的转染相当，我们使用1兆赫非聚焦超声以不同强度、脉冲重复频率和暴露时间对不同癌细胞系（U937、HeLa、PC-3、Meth A和T-24）进行超声处理。理想的超声条件为强度1.5瓦/平方厘米，脉冲频率0.5赫兹，占空比50%。结果表明，转染率随脉冲数增加而升高，在10至15个脉冲时达到峰值，之后开始下降。在本研究使用的固定条件下，利用这些最佳条件，我们已证明声孔效应转染优于电穿孔转染和脂质体介导的转染。这些发现表明，声孔效应转染可能是基因转染的其他非病毒方法（如电穿孔和脂质体介导的转染）的更好替代方法，尤其是在癌症基因治疗中。
