肠致病性大肠杆菌黏附因子质粒pMAR7的核苷酸序列分析
Nucleotide sequence analysis of the enteropathogenic Escherichia coli adherence factor plasmid pMAR7.
作者信息
Brinkley Carl, Burland Valerie, Keller Rogéria, Rose Debra J, Boutin Adam T, Klink Sara A, Blattner Frederick R, Kaper James B
机构信息
Department of Microbiology and Immunology, Center for Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore St., Baltimore, MD 21201, USA.
出版信息
Infect Immun. 2006 Sep;74(9):5408-13. doi: 10.1128/IAI.01840-05.
Abstract
The complete nucleotide sequence was determined for pMAR7, an enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid that contains genes encoding a type IV attachment pilus (Bfp) and the global virulence regulator per. Prototypic EAF plasmid pMAR7 is self-transmissible, unlike the smaller EAF plasmid pB171, which has no genes encoding conjugative functions. The tra locus, a highly conserved 33-kb segment found in pMAR7, is similar to the tra (conjugation) region of the F plasmid. ISEc13 copies flanking the pMAR7 tra region could potentially mobilize or delete the tra genes. Hybridization of 134 EPEC strains showed that a complete tra region is present only in strains of the EPEC1 clonal group. This study confirms EPEC's potential for dissemination of virulence attributes by horizontal transfer of the EAF plasmid.
摘要
测定了pMAR7的完整核苷酸序列，pMAR7是一种肠致病性大肠杆菌（EPEC）黏附因子（EAF）质粒，它包含编码IV型黏附菌毛（Bfp）和全局毒力调节因子per的基因。与较小的EAF质粒pB171不同，原型EAF质粒pMAR7是自我传递的，pB171没有编码接合功能的基因。在pMAR7中发现的tra位点是一个高度保守的33kb片段，与F质粒的tra（接合）区域相似。位于pMAR7 tra区域两侧的ISEc13拷贝可能会激活或删除tra基因。对134株EPEC菌株的杂交分析表明，完整的tra区域仅存在于EPEC1克隆群的菌株中。这项研究证实了EPEC通过EAF质粒水平转移传播毒力属性的可能性。
相似文献
1
Nucleotide sequence analysis of the enteropathogenic Escherichia coli adherence factor plasmid pMAR7.肠致病性大肠杆菌黏附因子质粒pMAR7的核苷酸序列分析
Infect Immun. 2006 Sep;74(9):5408-13. doi: 10.1128/IAI.01840-05.
2
A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence.一种与局部黏附相关的致病性大肠杆菌的质粒编码IV型菌毛基因。
Mol Microbiol. 1992 Nov;6(22):3427-37. doi: 10.1111/j.1365-2958.1992.tb02210.x.
3
Molecular evolution of typical enteropathogenic Escherichia coli: clonal analysis by multilocus sequence typing and virulence gene allelic profiling.典型肠致病性大肠杆菌的分子进化：多位点序列分型和毒力基因等位基因谱分析的克隆分析
J Bacteriol. 2007 Jan;189(2):342-50. doi: 10.1128/JB.01472-06. Epub 2006 Nov 10.
4
Complete DNA sequence and structural analysis of the enteropathogenic Escherichia coli adherence factor plasmid.肠致病性大肠杆菌黏附因子质粒的完整DNA序列及结构分析
Infect Immun. 1999 Oct;67(10):5455-62. doi: 10.1128/IAI.67.10.5455-5462.1999.
5
Cloning and characterization of the bundle-forming pilin gene of enteropathogenic Escherichia coli and its distribution in Salmonella serotypes.肠致病性大肠杆菌成束菌毛菌素基因的克隆、特性分析及其在沙门氏菌血清型中的分布
Mol Microbiol. 1993 Feb;7(4):563-75. doi: 10.1111/j.1365-2958.1993.tb01147.x.
6
Genetic analysis of enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid reveals a new deletion within the EAF probe sequence among O119 typical EPEC strains.对肠致病性大肠杆菌（EPEC）黏附因子（EAF）质粒的基因分析显示，在O119典型EPEC菌株的EAF探针序列内有一个新的缺失。
BMC Microbiol. 2015 Oct 5;15:200. doi: 10.1186/s12866-015-0539-9.
7
A cluster of fourteen genes from enteropathogenic Escherichia coli is sufficient for the biogenesis of a type IV pilus.来自肠致病性大肠杆菌的一组14个基因足以实现IV型菌毛的生物合成。
Mol Microbiol. 1996 Apr;20(2):325-37. doi: 10.1111/j.1365-2958.1996.tb02620.x.
8
Identification and characterization of the locus for diffuse adherence, which encodes a novel afimbrial adhesin found in atypical enteropathogenic Escherichia coli.弥漫性黏附位点的鉴定与特性分析，该位点编码一种在非典型肠致病性大肠杆菌中发现的新型无纤毛黏附素。
Infect Immun. 2005 Aug;73(8):4753-65. doi: 10.1128/IAI.73.8.4753-4765.2005.
9
Characterization of a Large Antibiotic Resistance Plasmid Found in Enteropathogenic Escherichia coli Strain B171 and Its Relatedness to Plasmids of Diverse E. coli and Shigella Strains.肠致病性大肠杆菌 B171 中发现的一种大抗生素抗性质粒的特性及其与不同大肠杆菌和志贺氏菌菌株质粒的相关性。
Antimicrob Agents Chemother. 2017 Aug 24;61(9). doi: 10.1128/AAC.00995-17. Print 2017 Sep.
10
Comparative sequence analysis of the plasmid-encoded regulator of enteropathogenic Escherichia coli Strains.肠致病性大肠杆菌菌株质粒编码调节因子的比较序列分析
Infect Immun. 2001 Sep;69(9):5553-64. doi: 10.1128/IAI.69.9.5553-5564.2001.
引用本文的文献
1
Cryo-EM structure and evolutionary history of the conjugation surface exclusion protein TraT.结合表面排斥蛋白TraT的冷冻电镜结构及进化史
Nat Commun. 2025 Jan 14;16(1):659. doi: 10.1038/s41467-025-55834-w.
2
Whole genome sequencing analysis of enteropathogenic from human and companion animals in Korea.韩国人和伴侣动物中致病性肠道细菌的全基因组测序分析
J Vet Sci. 2025 Jan;26(1):e1. doi: 10.4142/jvs.24225. Epub 2024 Dec 2.
3
Plasmids pick a bacterial partner before committing to conjugation.质粒在进行接合之前会选择一个细菌伙伴。
Nucleic Acids Res. 2023 Sep 22;51(17):8925-8933. doi: 10.1093/nar/gkad678.
4
The Interaction of the F-Like Plasmid-Encoded TraN Isoforms with Their Cognate Outer Membrane Receptors.F 样质粒编码的 TraN 同工型与其同源外膜受体的相互作用。
J Bacteriol. 2023 Apr 25;205(4):e0006123. doi: 10.1128/jb.00061-23. Epub 2023 Mar 29.
5
Mating pair stabilization mediates bacterial conjugation species specificity.配对稳定介导细菌接合种特异性。
Nat Microbiol. 2022 Jul;7(7):1016-1027. doi: 10.1038/s41564-022-01146-4. Epub 2022 Jun 13.
6
Atypical Enteropathogenic Escherichia coli: from Kittens to Humans and Beyond!非典型肠致病性大肠杆菌：从幼猫到人，乃至更远！
Infect Immun. 2021 Feb 16;89(3). doi: 10.1128/IAI.00752-20.
7
Novel Hybrid of Typical Enteropathogenic and Shiga-Toxin-Producing (tEPEC/STEC) Emerging From Pet Birds.源自宠物鸟的新型典型肠致病性和产志贺毒素（tEPEC/STEC）混合菌。
Front Microbiol. 2018 Dec 6;9:2975. doi: 10.3389/fmicb.2018.02975. eCollection 2018.
8
Environment Controls LEE Regulation in Enteropathogenic .环境控制肠道致病菌中的李氏菌调控
Front Microbiol. 2018 Jul 27;9:1694. doi: 10.3389/fmicb.2018.01694. eCollection 2018.
9
Self-Conjugation of the Enteropathogenic Adherence Factor Plasmid of Four Typical EPEC Isolates.四株典型 EPEC 致病粘附因子质粒的自身连接。
Biomed Res Int. 2017;2017:6325736. doi: 10.1155/2017/6325736. Epub 2017 Oct 26.
10
Classes 1 and 2 integrons in faecal Escherichia coli strains isolated from mother-child pairs in Nigeria.从尼日利亚母婴对中分离出的粪便大肠杆菌菌株中的1类和2类整合子。
PLoS One. 2017 Aug 22;12(8):e0183383. doi: 10.1371/journal.pone.0183383. eCollection 2017.
本文引用的文献
1
Pathogenic Escherichia coli.致病性大肠杆菌
Nat Rev Microbiol. 2004 Feb;2(2):123-40. doi: 10.1038/nrmicro818.
2
ASAP, a systematic annotation package for community analysis of genomes.ASAP，一个用于基因组群落分析的系统注释软件包。
Nucleic Acids Res. 2003 Jan 1;31(1):147-51. doi: 10.1093/nar/gkg125.
3
Typical and atypical enteropathogenic Escherichia coli.典型和非典型肠道致病性大肠杆菌
Emerg Infect Dis. 2002 May;8(5):508-13. doi: 10.3201/eid0805.010385.
4
The double par locus of virulence factor pB171: DNA segregation is correlated with oscillation of ParA.毒力因子pB171的双par位点：DNA分离与ParA的振荡相关。
Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15078-83. doi: 10.1073/pnas.261569598.
5
toxB gene on pO157 of enterohemorrhagic Escherichia coli O157:H7 is required for full epithelial cell adherence phenotype.肠出血性大肠杆菌O157:H7的pO157上的toxB基因是完全上皮细胞粘附表型所必需的。
Infect Immun. 2001 Nov;69(11):6660-9. doi: 10.1128/IAI.69.11.6660-6669.2001.
6
Comparative sequence analysis of the plasmid-encoded regulator of enteropathogenic Escherichia coli Strains.肠致病性大肠杆菌菌株质粒编码调节因子的比较序列分析
Infect Immun. 2001 Sep;69(9):5553-64. doi: 10.1128/IAI.69.9.5553-5564.2001.
7
Molecular variation among type IV pilin (bfpA) genes from diverse enteropathogenic Escherichia coli strains.不同肠道致病性大肠杆菌菌株IV型菌毛（bfpA）基因的分子变异
Infect Immun. 2000 Dec;68(12):7028-38. doi: 10.1128/IAI.68.12.7028-7038.2000.
8
Parallel evolution of virulence in pathogenic Escherichia coli.致病性大肠杆菌毒力的平行进化
Nature. 2000 Jul 6;406(6791):64-7. doi: 10.1038/35017546.
9
Complete DNA sequence and structural analysis of the enteropathogenic Escherichia coli adherence factor plasmid.肠致病性大肠杆菌黏附因子质粒的完整DNA序列及结构分析
Infect Immun. 1999 Oct;67(10):5455-62. doi: 10.1128/IAI.67.10.5455-5462.1999.
10
The Sinorhizobium meliloti insertion sequence (IS) element ISRm14 is related to a previously unrecognized IS element located adjacent to the Escherichia coli locus of enterocyte effacement (LEE) pathogenicity island.苜蓿中华根瘤菌插入序列（IS）元件ISRm14与一个先前未被识别的IS元件相关，该元件位于大肠杆菌肠细胞脱落位点（LEE）致病岛附近。
Curr Microbiol. 1999 Nov;39(5):274-81. doi: 10.1007/s002849900459.
Zotero 插件