Faculty of Health Sciences, Okayama University, 5-1 Shikata-cho 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
Int J Environ Res Public Health. 2022 Aug 26;19(17):10632. doi: 10.3390/ijerph191710632.
Typical indications for radon therapy include autoimmune diseases such as rheumatoid arthritis (RA). We had previously reported that radon inhalation inhibits Th17 immune responses in RA mice by activating Th1 and Th2 immune responses. However, there are no reports on how radon inhalation affects the activated Th1 and Th17 immune responses, and these findings may be useful for identifying new indications for radon therapy. Therefore, in this study, we investigated the effect of radon inhalation on the lipopolysaccharide (LPS)-induced inflammatory response, focusing on the expression of related cytokines and antioxidant function. Male BALB/c mice were exposed to 2000 Bq/m radon for one day. Immediately after radon inhalation, LPS was administered intraperitoneally at 1.0 mg/kg body weight for 4 h. LPS administration increased the levels of Th1- and Th17-prone cytokines, such as interleukin-2, tumor necrosis factor-α, and granulocyte-macrophage colony-stimulating factor, compared to no treatment control (sham). However, these effects were suppressed by radon inhalation. IL-10 levels were significantly increased by LPS administration, with or without radon inhalation, compared to sham. However, radon inhalation did not inhibit oxidative stress induced by LPS administration. These findings suggest that radon inhalation has immunomodulatory but not antioxidative functions in LPS-induced injury.
氡治疗的典型适应证包括类风湿关节炎（RA）等自身免疫性疾病。我们之前曾报道过，氡吸入通过激活 Th1 和 Th2 免疫应答来抑制 RA 小鼠的 Th17 免疫应答。然而，目前尚无关于氡吸入如何影响激活的 Th1 和 Th17 免疫应答的报道，这些发现可能有助于确定氡治疗的新适应证。因此，在这项研究中，我们研究了氡吸入对脂多糖（LPS）诱导的炎症反应的影响，重点研究了相关细胞因子的表达和抗氧化功能。雄性 BALB/c 小鼠暴露于 2000 Bq/m 的氡气中一天。氡吸入后立即，以 1.0 mg/kg 体重的 LPS 腹膜内给药 4 h。与未处理对照组（假处理）相比，LPS 给药增加了 Th1 和 Th17 倾向细胞因子的水平，如白细胞介素-2、肿瘤坏死因子-α和粒细胞-巨噬细胞集落刺激因子。然而，这些效应被氡吸入抑制。与假处理相比，LPS 给药会显著增加白细胞介素-10 水平，而氡吸入与否。然而，氡吸入并不能抑制 LPS 给药引起的氧化应激。这些发现表明，氡吸入在 LPS 诱导的损伤中具有免疫调节作用，但没有抗氧化作用。
