酶法制备的RNA干扰文库。

Enzymatically prepared RNAi libraries.

作者信息

Buchholz Frank, Kittler Ralf, Slabicki Mikolaj, Theis Mirko

机构信息

Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany.

出版信息

Nat Methods. 2006 Sep;3(9):696-700. doi: 10.1038/nmeth912.

DOI:10.1038/nmeth912

PMID:16929314

Abstract

Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.

摘要

在哺乳动物细胞中进行的大规模RNA干扰（RNAi）筛选主要使用合成的小干扰RNA（siRNA）或短发夹RNA（shRNA）文库。这两种方法的RNAi触发因子都是通过基于算法的预测设计的，以识别用于敲低mRNA的单个序列。最近已经开发出了使用酶促方法的这些方法的替代方案。在这里，我们描述了酶促制备的shRNA和siRNA文库的概念，并讨论了它们的优点和局限性。