Zanatta Leila, de Sousa Eliandra, Cazarolli Luisa Helena, Junior Anildo Cunha, Pizzolatti Moacir Geraldo, Szpoganicz Bruno, Silva Fátima Regina Mena Barreto
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Cx. Postal 5069, CEP 88040-970-Florianópolis, SC, Brazil.
J Ethnopharmacol. 2007 Jan 3;109(1):151-5. doi: 10.1016/j.jep.2006.07.019. Epub 2006 Jul 22.
The effect of the crude extract, ethyl acetate and n-butanol fractions from Vitex megapotamica (Spreng) Moldenke on glycemia was investigated in diabetic rats. Oral administration of crude extract significantly reduced serum glucose levels in both normal and diabetic animals. In normal rats, serum glucose lowering was observed with 400 and 800 mg/kg at 2 and 2-3h, respectively after oral crude extract treatment. Nevertheless, the hypoglycemic effect of Vitex megapotamica in diabetic rats was evident at 1 and 2h and from 1 to 3h after treatment with 400 and 800 mg/kg, respectively. The ethyl acetate as well as n-butanol fractions were able to diminish glycemia in diabetic animals. The ethyl acetate fraction (400 and 800 mg/kg) produced the maximum hypoglycemic effect (28 and 20%, respectively) in diabetic rats and the same dose of the n-butanol fraction reduced the hyperglycemia only by 11% at 1h after treatment. Additionally, in hyperglycemic normal rats neither crude extract nor ethyl acetate fraction modified the glucose tolerance and the known tolbutamide effect on insulin release was clearly observed in this group. Thus, this study shows that Vitex megapotamica has an anti-hyperglycemic action, is able to ameliorate the diabetic state and, probably, is a source of hypoglycemic compounds.
研究了大果牡荆（Vitex megapotamica (Spreng) Moldenke）的粗提物、乙酸乙酯部位和正丁醇部位对糖尿病大鼠血糖的影响。口服粗提物可显著降低正常和糖尿病动物的血清葡萄糖水平。在正常大鼠中，口服粗提物后，分别在2小时和2 - 3小时观察到400和800 mg/kg剂量的血清葡萄糖降低。然而，大果牡荆对糖尿病大鼠的降血糖作用在分别给予400和800 mg/kg治疗后1小时和2小时以及1至3小时时明显。乙酸乙酯部位和正丁醇部位均能降低糖尿病动物的血糖。乙酸乙酯部位（400和800 mg/kg）在糖尿病大鼠中产生最大降血糖作用（分别为28%和20%），相同剂量的正丁醇部位在治疗后1小时仅使高血糖降低11%。此外，在高血糖正常大鼠中，粗提物和乙酸乙酯部位均未改变葡萄糖耐量，且该组未明显观察到已知的甲苯磺丁脲对胰岛素释放的作用。因此，本研究表明大果牡荆具有抗高血糖作用，能够改善糖尿病状态，并且可能是降血糖化合物的来源。
