Norman M E, Elgendy H M, Shors E C, el-Amin S F, Laurencin C T
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, USA.
Clin Mater. 1994;17(2):85-91. doi: 10.1016/0267-6605(94)90016-7.
The purpose of this study was to determine the potential of coralline calcium phosphate ceramics to support osteoblast growth for a proposed bone-ceramic composite for skeletal tissue repair. The goal was the development of a matrix with both osteogenic and osteoconductive properties, as compared to ceramic alone, which is solely osteoconductive. MC3T3-E1 osteoblast-like cells were seeded onto sintered and non-sintered porous coralline hydroxyapatite (HA), and onto non-porous hydroxyapatite discs. These in-vitro studies demonstrated that coralline HA supported the growth of osteoblast-like cells. Porous discs supported higher numbers of cells than non-porous discs. Sintering encouraged cell growth, with higher numbers of cells adhered to sintered porous HA discs by day seven. The results suggest that HA can provide a support for osteoblast cells as part of a matrix which may prove to be osteogenic in vivo and may, accordingly, enhance the bone repair process.
本研究的目的是确定珊瑚磷酸钙陶瓷作为一种用于骨骼组织修复的骨-陶瓷复合材料，支持成骨细胞生长的潜力。目标是开发一种兼具成骨和骨传导特性的基质，与仅具有骨传导性的单一陶瓷相比。将MC3T3-E1成骨样细胞接种到烧结和未烧结的多孔珊瑚羟基磷灰石（HA）上，以及无孔羟基磷灰石圆盘上。这些体外研究表明，珊瑚HA支持成骨样细胞的生长。多孔圆盘比无孔圆盘支持更多数量的细胞。烧结促进细胞生长，到第7天时，烧结多孔HA圆盘上附着的细胞数量更多。结果表明，HA作为基质的一部分可为成骨细胞提供支持，这种基质在体内可能具有成骨作用，因此可能会增强骨修复过程。
