Hassani-Mehraban A, Saaijer J, Peters D, Goldbach R, Kormelink R
Laboratory of Virology, Wageningen University, Wageningen, The Netherlands.
Arch Virol. 2007 Jan;152(1):85-96. doi: 10.1007/s00705-006-0827-7. Epub 2006 Aug 21.
Two strains of Tomato yellow ring virus (TYRV, genus Tospovirus), one from tomato (referred to as TYRV-t) and the other from soybean and potato (denoted TYRV-s), collected from different geographical regions in Iran, were compared. Their genomic S RNA segments differed in size by 55 nucleotides. Comparison of the S RNA intergenic regions revealed the absence of a stretch of 115 nucleotides within the S RNA segment of TYRV-s and, conversely, of 56 nts in that of TYRV-t, apparently a stable genetic difference as it was also found in another isolate of TYRV-s collected from potato. Sequence comparison of the N protein ORFs revealed an identity of 92% between the N proteins of both strains, and the observed strong cross-reaction of TYRV-s in DAS-ELISA with a polyclonal antiserum directed against the TYRV-t N protein confirmed this high identity. Host range analysis revealed several differences, e.g. TYRV-s, but not TYRV-t, being able to systemically infect Nicotiana species, and TYRV-s being localised in tomato. The observed molecular and biological differences of both viruses call into question the currently used criteria for Tospovirus species demarcation.
对从伊朗不同地理区域收集的两株番茄黄环病毒（TYRV，番茄斑萎病毒属）进行了比较，一株来自番茄（称为TYRV-t），另一株来自大豆和马铃薯（记为TYRV-s）。它们的基因组S RNA片段大小相差55个核苷酸。对S RNA基因间隔区的比较显示，TYRV-s的S RNA片段中缺少一段115个核苷酸的序列，相反，TYRV-t的S RNA片段中缺少56个核苷酸，这显然是一种稳定的遗传差异，因为在从马铃薯中分离的另一株TYRV-s中也发现了这种差异。N蛋白开放阅读框的序列比较显示，两株病毒的N蛋白之间的同一性为92%，并且在DAS-ELISA中观察到TYRV-s与针对TYRV-t N蛋白的多克隆抗血清有强烈的交叉反应，证实了这种高度同一性。寄主范围分析揭示了一些差异，例如，TYRV-s能够系统感染烟草属植物，而TYRV-t不能，并且TYRV-s局限于番茄中。观察到的两种病毒在分子和生物学上的差异对目前用于番茄斑萎病毒属物种划分的标准提出了质疑。
